Human Nuclear Autoantibody (IgG) ELISA kit (CAT#: NAK-008)

This product is a qualitative ELISA kit for the determination of Nuclear Autoantibody in human Plasma or Serum.

Specifications
SensitivityQualitative
Assay TypeELISA
Pack size1×96 wells
Species ReactivityHuman
Antibody TypeIgG
ContentsHRP-conjugate
Positive Control
Negative Control
Adhesive Strip (For 96 wells)
Coated assay plate
Wash Buffer (25 x concentrate)
Stop Solution
Sample Diluent
Substrate A
Substrate B
Sample RequirementSerum
StorageShort term: 4°C, Long term: See manual.
Target Information
TargetNuclear autoantibody
IntroductionThe purpose of an antinuclear antibody test is to detect, measure, and evaluate antinuclear antibodies in a patient's blood sample. ANA testing can assist health care providers in diagnosing autoimmune disorders and provide information that may be helpful in determining a patient's specific type of autoimmune disorder.
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Q: What is the main application of the Human Nuclear Autoantibody (IgG) ELISA kit?
A: The main application of the Human Nuclear Autoantibody (IgG) ELISA kit is the qualitative detection of nuclear autoantibodies in human plasma or serum. This is pivotal in the clinical diagnosis and management of autoimmune disorders. By identifying the presence of antinuclear antibodies (ANAs), health care providers can better understand the autoimmune activities in patients, aiding in the diagnosis of conditions like systemic lupus erythematosus (SLE), rheumatoid arthritis, and other autoimmune diseases. This kit's ability to provide qualitative results is crucial for the early detection and intervention in these conditions.

Q: How does the Human Nuclear Autoantibody ELISA kit work?
A: The Human Nuclear Autoantibody ELISA kit works by using a coated assay plate where samples of human serum or plasma are added. These samples react with the antigens coated on the plate if nuclear autoantibodies are present. The reaction is then detected using an HRP-conjugate, which binds to the autoantibodies. Substrates A and B are added, leading to a color change that indicates the presence of nuclear autoantibodies. Positive and negative controls are used to ensure the assay's accuracy, with the results being qualitative.

Q: What are the contents included in the Human Nuclear Autoantibody (IgG) ELISA kit?
A: The Human Nuclear Autoantibody (IgG) ELISA kit contains components such as HRP-conjugate, Positive and Negative Control, Adhesive Strip for 96 wells, Coated assay plate, Wash Buffer (25 x concentrate), Stop Solution, Sample Diluent, Substrate A, and Substrate B.

Q: Why is it important to detect nuclear autoantibodies?
A: Detecting nuclear autoantibodies is crucial because their presence is often associated with a variety of autoimmune disorders. These antibodies target the body's own nuclear materials, leading to inflammation and damage to various organs and tissues. Identifying these autoantibodies helps healthcare providers diagnose conditions such as systemic lupus erythematosus, Sjogren's syndrome, scleroderma, and rheumatoid arthritis. Early detection through testing can lead to timely intervention, potentially improving patient outcomes by managing symptoms and preventing severe disease progression.

Excellent sensitivity and reactivity
The Human Nuclear Autoantibody ELISA kit provided excellent sensitivity and reactivity for the detection of Nuclear Autoantibody in human plasma or serum. All the necessary components are provided in the kit, which is very convenient and makes the procedure straightforward. The assay was qualitative and gave reliable results each time. The storage conditions are also simple, allowing for short term storage at 4°C, and for the long term, you just need to refer to the manual. Highly recommended!


02/20/2023

Streamlined workflow for autoimmune research
In our laboratory, the Human Nuclear Autoantibody (IgG) ELISA kit has significantly streamlined our research on autoimmune disorders. The kit's user-friendly components and clear instructions have reduced our assay setup time, allowing us to process samples more efficiently. The coated assay plate, combined with the high-quality HRP-conjugate, wash buffer, and substrates, ensures precise and accurate measurements every time. Our research has benefitted greatly from the kit's qualitative sensitivity, enabling us to confidently analyze the prevalence and significance of nuclear autoantibodies in our studies.


04/22/2023

Confident and consistent results
The kit provides all the necessary components, ensuring the accuracy of assays. With it being highly species-specific to humans, the product has always provided confident results for all my tests. And with proper storage, the kit consistently delivers high-quality outcomes. It's an essential tool in evaluating antinuclear antibodies, providing vital information for our autoimmune disorder studies.


01/25/2024

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Title: Distribution of IgG Subclass Anti-nuclear Antibodies (ANAs) in Systemic Lupus Erythematosus

Journal: Lupus

Authors: Yanli Zeng, Yan Zhang, Qinggui Chen, et al.

Abstract
For Research Use Only | Not For Clinical Use

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