Human Oxidized LDL Autoantibody (IgG) ELISA kit (CAT#: NAK-004)

This product is an ELISA kit for the determination of Oxidized LDL Autoantibody in human Plasma or Serum. It is highly sensitive and available for the detection of Oxidized LDL Autoantibody as low as 48 mU/ml.

Specifications
Sensitivity48 mU/ml
Assay TypeIndirect ELISA
Pack size1×96 wells
Species ReactivityHuman
Antibody TypeIgG
ContentsOxidized LDL precoated microtiter strips in strip holder
Uncoated microtiter plate
Wash buffer concentrate 20x
Standards 1-6
Control A and B
Assay buffer
Conjugate
Substrate (TMB solution)
Stop solution
Sample RequirementSerum: 50 ul
StorageShort term: 4°C, Long term: See manual.
Target Information
TargetOxidized LDL autoantibody
IntroductionOxidized low-density lipoprotein (LDL) is a potentially harmful type of cholesterol that is produced in the body when normal LDL cholesterol is damaged by chemical interactions with free radicals. Together with inflammatory responses, free radicals can result in hardening of the arteries (atherosclerosis).
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Q: What is the Human Oxidized LDL Autoantibody (IgG) ELISA Kit used for?
A: The Human Oxidized LDL Autoantibody (IgG) ELISA Kit (CAT#: NAK-004) is designed for the precise determination of oxidized LDL autoantibodies in human plasma or serum. These autoantibodies are crucial for understanding atherosclerosis as oxidized LDL plays a significant role in arterial plaque formation and cardiovascular disease.

Q: How sensitive is the Human Oxidized LDL Autoantibody ELISA Kit?
A: This ELISA kit boasts high sensitivity, capable of detecting oxidized LDL autoantibodies with a minimum detectable level of 48 mU/ml. This ensures that even low concentrations of autoantibodies can be quantified, providing accurate and reliable results for researchers studying cardiovascular diseases.

Q: What sample volume is required for the Human Oxidized LDL Autoantibody ELISA Kit and how should samples be stored?
A: A 50 µl serum sample is needed for the assay. For short-term storage, samples and kit components should be kept at 4°C. For long-term storage instructions, users should refer to the manual to maintain the kit's integrity and stability for consistent results.

Q: What is the significance of detecting Oxidized LDL autoantibodies?
A: Oxidized LDL autoantibodies serve as biomarkers for oxidative stress and are associated with the progression of atherosclerosis. By determining the presence and level of these autoantibodies, researchers can gain insight into the pathophysiological mechanisms of atherosclerosis and related inflammatory responses within the cardiovascular system.

Exceptional sensitivity and reliability
I've been utilizing the Human Oxidized LDL Autoantibody (IgG) ELISA kit for several months now in my cardiovascular research. The sensitivity of this kit has significantly enhanced the accuracy of my studies. The detailed components, including the Oxidized LDL precoated microtiter strips and a comprehensive range of standards and controls, provided consistent and reliable results. This kit is a staple in my laboratory for its unwavering quality and reproducibility.


04/29/2022

Value for money with superior performance
Considering the superior sensitivity, comprehensive kit contents, and ease of use, the Human Oxidized LDL Autoantibody (IgG) ELISA kit offers great value for money. The inclusion of detailed components such as precoated strips, a variety of standards, and controls, allows for a wide range of applications in studying atherosclerosis and oxidative stress. Its performance in our lab has been nothing short of exceptional, providing accurate and reproducible results consistently. For any lab looking to invest in a high-quality ELISA kit for cardiovascular research, this is the product to go for.


01/14/2023

High quality with great species reactivity
In our quest to understand the implications of oxidized LDL in atherosclerosis, this ELISA kit has been instrumental. The specificity to human IgG and the high sensitivity of the assay have allowed us to detect nuanced changes in oxidized LDL autoantibody levels in patient samples. What impressed me most was the kit's species reactivity, ensuring that our human serum and plasma samples provided reliable and consistent data. This is a high-quality kit that I would suggest to any researcher focusing on cardiovascular diseases.


07/23/2023

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Title: A Novel Immunoassay for Malondialdehyde-Conjugated Low-Density Lipoprotein Measures Dynamic Changes in the Blood of Patients Undergoing Coronary Artery Bypass Graft Surgery

Journal: Antioxidants

Authors: Samata S. Pandey, Adam Hartley, Mikhail Caga-Anan, et al.

Abstract
For Research Use Only | Not For Clinical Use

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