Human SSB Autoantibody (IgG) ELISA kit (CAT#: NAK-007)

This product is a qualitative ELISA kit for the determination of SSB Autoantibody in human Plasma or Serum.

Specifications
Sensitivity	Qualitative
Assay Type	ELISA
Pack size	1×96 wells
Species Reactivity	Human
Antibody Type	IgG
Contents	Microwells coated with SSB antigen Sample Diluent Calibrator Positive Control Negative Control Enzyme conjugate TMB Substrate Stop Solution Wash concentrate 20X
Sample Requirement	Plasma, Serum
Storage	Short term: 4°C, Long term: See manual.

Target Information
Target	SSB autoantibody
Alternative Names	Small RNA Binding Exonuclease Protection Factor La autoantibody; Lupus La Protein autoantibody; La Autoantigen autoantibody; La/SSB autoantibody; LARP3 autoantibody; La autoantibody; La Ribonucleoprotein Domain Family, Member autoantibody; Sjoegren Syndrome Type B Antigen autoantibody; Sjogren Syndrome Antigen B autoantibody; SS-B autoantibody; Sjogren Syndrome Antigen B (Autoantigen La) autoantibody; La Ribonucleoprotein autoantibody; Lupus La Antigen autoantibody; SS-B/La Protein autoantibody; Autoantigen La autoantibody
Introduction	The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.

Q&A
Customer Reviews
Downloadable Resources
Published Data

Q: What is the purpose of the Human SSB Autoantibody (IgG) ELISA kit and its significance?
A: The Human SSB Autoantibody (IgG) ELISA kit is designed for the qualitative determination of SSB autoantibodies in human plasma or serum. It holds significant importance in clinical diagnostics and research due to its role in identifying autoantibodies associated with Sjogren syndrome and systemic lupus erythematosus (SLE). Detecting these autoantibodies is crucial for the diagnosis and management of these autoimmune disorders, as they can indicate an immune response against the body's own proteins involved in RNA metabolism, potentially leading to various systemic complications.

Q: What are the contents and requirements of the kit?
A: The kit contains microwells coated with SSB antigen, sample diluent, a calibrator, a positive control, a negative control, an enzyme conjugate, the TMB substrate, a stop solution, wash concentrate 20X. The sample requirement for this kit is plasma or serum.

Q: How should the ELISA kit be stored and what is its reactivity?
A: The ELISA kit should be stored at 4°C for short term usage and for the long term storage conditions, one should refer to the manual. It exhibits species reactivity with humans.

Q: Can this ELISA kit detect SSB autoantibodies in all types of human plasma and serum?
A: Yes, the Human SSB Autoantibody (IgG) ELISA kit is designed to work with all types of human plasma and serum. Its compatibility with diverse sample types makes it a versatile tool for detecting SSB autoantibodies in a wide range of clinical and research settings. This flexibility ensures that the kit can be used effectively across various diagnostic laboratories and research facilities, providing reliable qualitative results irrespective of the plasma or serum source.

High-quality and reliable SSB autoantibody detection
This is an easy-to-use, quick, and reliable kit for detecting SSB Autoantibodies in human serum and plasma. I found it to have excellent specificity and sensitivity. The quality of each component in the kit is appreciable. Highly recommend it to anyone in need of accurate and consistent results.

09/21/2022

Enhancing autoimmune research with specificity and ease
Our laboratory recently utilized the Human SSB Autoantibody (IgG) ELISA kit in a series of tests aimed at understanding the prevalence of SSB autoantibodies in various autoimmune conditions. The specificity of the kit for human IgG and its user-friendly components significantly enhanced our workflow and data reliability. Particularly noteworthy is the kit's qualitative sensitivity, which enabled us to confidently categorize our samples. The detailed manual and the inclusion of a comprehensive set of reagents and controls have made it an indispensable tool in our autoimmune disease research.

03/14/2023

Unmatched accuracy and quality
Impressively accurate! I've used this ELISA kit in my lab and it has never failed me. It reacts well with Human samples and the protocol is easy to follow. The contents of the kit are all of high quality and well packaged. The added positive and negative controls are useful for validation.

08/29/2023

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Datasheet NAA Detection Kits Flyer

Title: The Interaction Between Anti-Ro/SSA and Anti-La/SSB Autoantibodies and Anti-Infectious Antibodies in A Wide Spectrum of Auto-Immune Diseases: Another Angle of The Autoimmune Mosaic

Journal: Clinical and Experimental Rheumatology

Authors: N. Agmon-Levin, A. Dagan, Y. Peri, et al.

Objective: The presence of anti-Ro/SSA and anti-La/SSB antibodies has been linked with autoimmunity in general and with several autoimmune diseases (AID) in particular. In the current study we evaluated these antibodies in a wide spectrum of AID as well as the links between them and anti-infectious antibodies.

Methods: We examined 2082 sera from patients with 16 different AID compared to 524 sera from geographically-matched healthy controls, for the presence and titres of anti-Ro/SSA and anti-La/SSB. All samples were also tested for a variety of anti-infectious agents' antibodies using the BioPlex 2200-immunoassay (Bio-Rad, USA).

Results: Anti-Ro/SSA was more prevalent, with significantly higher titre in 5 autoimmune diseases namely Sjögren's syndrome (SS), systemic lupus erythematosus (SLE), antiphospholipid syndrome (APS) both primary and APS linked to SLE, systemic sclerosis (SSc) and primary biliary cirrhosis (PBC). Anti-La/SSB was more prevalent with higher titers in SS, SLE, APS linked to SLE and PBC. Prevalence, but not titers, of both antibodies were higher also in polymyositis (PM). Additionally, we found a correlation between anti-Ro/SSA antibodies and antibodies of the IgM and IgG subtypes directed at cytomegalovirus as well as IgG-antibodies directed at Epstein-Barr virus (EBV) and toxoplasma (p<0.001). Anti-La/SSB antibodies correlated with the presence of IgG antibodies against EBV early antigen (p<0.001).

Conclusion: In a large cohort of patients with autoimmune diseases we found an association between anti-Ro/SSA and anti-La/SSB antibodies and 6 autoimmune diseases, amongst which primary APS and PM. Additionally, we observed linkages between these autoantibodies and anti-infectious antibodies directed at Epstein-Barr virus, toxoplasma and cytomegalovirus. Our findings support the concept of interplay between infectious agents and autoimmunity, such as the plausibility of an infectious agent that trigger the immune system to produce specific antibodies which will later result in a unique group of AID.

Titre of anti La/SSB in various autoimmune diseases.

For Research Use Only | Not For Clinical Use

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