Ribosome Display System Overviews

Ribosome display technology mainly refers to mRNA, proteins, and ribosomes being connected and forming a protein-ribosome-mRNA ternary complex in the form of non-covalent bonds, and then finally applied to substrate screening. This in vitro display technology can overcome some of the shortcomings of the in vivo display system, and it can be more easily screened for the target molecules. Therefore, ribosome display technology is widely used in the screening of antibodies, proteins, and peptides. Based on our rich field experience and ribosome display platform, Creative Biolabs provides comprehensive services to support the development and application of ribosome display technology.

Fig 1. Ribosome. (Creative Biolabs Authorized)

Ribosome Display

Ribosome display technology is performed in vitro and avoids some of the limitations of cell-based display systems. By forming mRNA-ribosome-protein trimers in a cell-free system, nascent proteins are associated with their associated mRNA molecules, and the genetic material is selected by the functional properties of the proteins. mRNAs can be amplified or reverse-transcribed by RT-PCR into DNA, which can then be recycled, mutated, or cloned. In recent years, researchers have utilized ribosome display technology for extensive studies and have improved the traditional ribosome display technology to successfully screen a wide range of macromolecules, such as antibodies, enzymes, peptides, and some small molecules that have a specific affinity for target molecules.

At present, based on ribosome display technology and combined with practical applications, scholars have developed many unique ribosome display technologies, such as ribosome inactivation display system, prokaryotic ribosome display system, and eukaryotic ribosome display system.

The ribosome inactivation display system is an important method for in vitro protein evolution. The protein-ribosome-mRNA ternary complexes obtained by the ribosome inactivation display system are more stable than those obtained by common ribosome display techniques. In addition, the ribosome inactivation display system can screen functional proteins under standard translation conditions without removing stop codons.

Ribosome display technology is a complete in vitro display system that can overcome problems that cannot be solved by other display technologies. First, ribosome display technology has the advantage of large library capacity. Ribosome display technology does not require living cells, which avoids the strict control of conditions required in in vivo screening. It does not involve cell transfection and therefore is not subject to constraints such as transformation efficiency, phage packaging, transmembrane secretion, and protease degradation. The screening results of molecular libraries are positively correlated with the library capacity, so it is easier to screen target molecules with large-capacity ribosome display technology. The biggest disadvantage of ribosome display technology is that mRNA is easy to degrade. mRNA can effectively maintain the stability of 15 d, but due to the RNA is easy to be hydrolyzed and enzymatically degraded. Therefore, the stability of mRNA is still the main defect of in vitro ribosome display system. In addition, generally speaking, protein-ribosome-mRNA ternary complexes are less stable, and protein-ribosome-mRNA ternary complexes must be kept intact to ensure the smooth progress of the display system. The ribosome inactivation display system can make up for this defect to a certain extent to obtain more stable protein-ribosome-mRNA ternary complexes.

More Details on Ribosome Display System

Ribosome-Inactivation Display System Introduction
Prokaryotic Ribosome Display System Introduction
Eukaryotic Ribosome Display System Introduction
Introduction to In Vitro Antibody Evolution by Ribosome Display
Introduction to In Vitro Protein Evolution by Ribosome Display

Creative Biolabs has a wealth of knowledge and experience in ribosome display systems. We would be happy to share with you our knowledge and experience on ribosome display for in vitro antibody evolution and in vitro protein evolution.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: