SLC30A3 Membrane Protein Introduction

Introduction of SLC30A3

Solute carrier family 30 member 3 (SLC30A3), also known as zinc transporter 3 (ZnT-3), is a protein that in humans is encoded by the SLC30A3 gene. As a member of the SLC30A family, ZnT-3 plays a key role in the accumulation of zinc ions inside synaptic vesicles.

Function of SLC30A3 Membrane Protein

SLC30A3 or ZnT3 is a putative transporter of zinc into synaptic vesicles of neurons and it belongs to the zinc transporter family. With six transmembrane domains enclosing a pore lined with a histidine-rich loop, ZnT3 shares 44% homology with ZnT8 which is essential for insulin crystallization in pancreatic beta-cells. It has been reported that the human-ZnT3 protein has 388 amino acids and shares 86 and 87% homologies with rat- and mouse-Znt3, respectively. During the past years, studies have shown that the expression of ZnT3 is restricted to tissues of brain and testis. The SLC30A3 expression has also been detected in the epithelial cells of the choroid plexus, the Bergman glial cells of mouse cerebellar cortex and the postganglionic neurons of mouse superior cervical ganglia. What’s more, the expression of SLC30A3 is age-dependant at least in the cerebral cortex and in pancreatic islets.

Fig.1 The topology of ZnT3. (Smidt, 2012)

Application SLC30A3 of Membrane Protein in Literature

1. Choi B.Y., et al. ZnT3 Gene deletion reduces colchicine-induced dentate granule cell degeneration. International journal of molecular sciences. 2017, 18(10):2189. PubMed ID: 29048371
   
   

   This study aims to identify the role of ZnT3 gene deletion on colchicine-induced dentate granule cell death. It suggests that increased neuronal glutathione by ZnT3 gene deletion prevents colchicine-induced dentate granule cell death.

2. Kurita H., et al. Protective roles of SLC30A3 against endoplasmic reticulum stress via ERK1/2 activation. Biochemical and biophysical research communications. 2016, 479(4):853-9. PubMed ID: 27678294
   
   

   This article investigates the role of SLC30A3 against endoplasmic reticulum (ER) stress. It suggests that SLC30A3 may play a protective role against ER stress, which is related to ERK1/2 activation.

3. Choi B.Y., et al. Zinc transporter 3 (ZnT3) gene deletion reduces spinal cord white matter damage and motor deficits in a murine mog-induced multiple sclerosis model. Neurobiology of disease. 2016, 94:205-12. PubMed ID: 27370228
   
   

   This article aims to evaluate the role of zinc transporter 3 (ZnT3) on multiple sclerosis (MS) pathogenesis. It demonstrates that ZnT3 gene deletion could inhibit the clinical features and neuropathological changes associated with experimental autoimmune encephalomyelitis.

4. Smidt K., et al. The zinc transporter ZNT3 co-localizes with insulin in INS-1E pancreatic beta cells and influences cell survival, insulin secretion capacity, and ZNT8 expression. Biometals. 2016, 29(2):287-98. PubMed ID: 26867900
   
   

   This article demonstrates that ZNT3 and ZNT8 (known to regulate insulin secretion) have opposite effects on insulin synthesis and secretion possibly by a transcriptional co-regulation since mRNA expression of ZNT3 is inversely correlated to ZNT8 and ZNT3 over-expression reduced insulin synthesis and secretion in INS-1E cells. Furthermore, ZNT3 over-expression could improve cell survival.

5. Sopian N.F., et al. Does short-term dietary omega-3 fatty acid supplementation influence brain hippocampus gene expression of zinc transporter-3? International journal of molecular sciences. 2015, 16(7):15800-10. PubMed ID: 26184176
   
   

   The objective of this study is to explore the effect of short-term dietary omega-3 fatty acids on hippocampus gene expression at the molecular level in relation to spatial recognition memory in mice. Results suggest that the increased ZnT3 levels caused by the deficiency of omega-3 fatty acids produce an abnormal zinc metabolism that in turn impairs the brain cognitive performance in mice.

SLC30A3 Preparation Options

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Reference

1. Smidt, et al. (2012). ZnT3: a zinc transporter active in several organs. Biometals. 25(1): 1-8.

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