Solid-Phase Strategy based Library Screening Service

Background Service FAQ Resources

Creative Biolabs is a well-recognized service provider of phage display technology. We have developed a serial of advanced phage display library screening methods for selecting antibodies with high affinity and specificity. Solid-phase screening is a valuable method that is rapid, efficient and relatively inexpensive for selecting the binding antibodies, identifying epitopes, etc. We offer an optimized solid-phase screening strategy which guarantees the success of finding binders targeting various types of antigens.

Background

Phage display is one of the most effective technology for antibody generation since it links the phage phenotype and its encapsulated genotype. In general, the peptide or antibody library is constructed by fusing interested proteins with pIII, pVIII or other capsid proteins and displaying on the surface of the M13 phage particle. Our seasoned scientists are confident in generating high-quality libraries with diversity over 10¹⁰.

Phage display library has become a popular tool for treating autoimmune and cancer diseases. While the biopanning stage plays a crucial role in the enrichment and isolation of desired binders that can effectively recognize the interested targets. This stage involves a repeated cycles of incubation, washing, elution and amplification. Multiple rounds of selection cycle are required for obtaining monoclonal phage antibodies with desired binding affinity. Enzyme-linked immunosorbent assay (ELISA) could also be incorporated to determine the activity after the selection. As the most widely used biopanning method, solid-phase screening indicates an effective selection approach that can apply to most of binder selection projects. A series of solid-phase, like microtiter plate wells, PVDF membranes, could be used for target molecule immobilization.

Fig.1 Process of phage display library selection.

Several components in the screening system affect the selection result, such as the type of solid support, time of binding, washing and elution, and antigen concentration. We have developed an optimized procedure which minimizes the possibility of selecting target-unrelated binders. In order to reduce the binding to plastic, several methods could be applied, such as blocking the surface or immobilizing the target using an alternative approach other than direct adsorption. The density of the target could be increased to reduce the possibility of selecting background binders. Also, subtractive selection could be used to remove background binders.

Solid-Phase Strategy based Library Screening Service

Creative Biolabs is highly experienced in phage display technology and has proven records of generating high-affinity antibodies in a cost-effective and timely manner. Our solid-phase screening is optimized and precisely controlled, therefore avoiding the possibility of false-positives. Combined with other advanced technologies, we could offer the most comprehensive services and products based on our phage display platform, from immunization, library construction and screening to antibody engineering. Our scientists are pleased to offer tailored solutions to meet our customers' different projects.

Other optional phage display library screening services:

FAQ

What is solid-phase phage display library screening
Solid-phase phage display library screening is a technique used to identify peptides, proteins, or antibodies that bind to a specific target immobilized on a solid surface. Phages displaying various peptides are introduced to the target, and those that bind are retained, while non-binders are washed away. The process identifies high-affinity binding molecules that can be further studied or developed into therapeutic agents.
How does solid-phase phage display screening work
In solid-phase phage display screening, the target molecule is immobilized on a solid surface such as a microplate or bead. A phage display library containing millions of phages with different peptide sequences is incubated with the target. Phages that bind to the target remain on the surface, while unbound phages are washed away. Bound phages are then eluted and amplified for further rounds of selection.
What are the advantages of using solid-phase phage display over other methods
Solid-phase phage display offers high throughput and sensitivity, allowing for the screening of large peptide libraries against immobilized targets. The solid-phase format simplifies washing steps and ensures that only high-affinity binders are retained. Additionally, this method allows for the easy modification of the solid surface to accommodate different targets, making it versatile for various applications.
What are the key applications of solid-phase phage display screening
Solid-phase phage display is widely used in drug discovery, antibody development, and the identification of protein-protein interactions. It helps researchers find peptides or antibodies that bind to disease-related targets, enabling the development of new therapies. This method is also employed in diagnostics, vaccine development, and the creation of biopharmaceuticals by identifying high-affinity binding molecules.
What types of targets can be used in solid-phase phage display screening
Various types of targets can be used in solid-phase phage display screening, including proteins, peptides, nucleic acids, and small molecules. The target is typically immobilized on a solid surface, and the phage display library is screened against it to identify binding interactions. This versatility makes solid-phase phage display applicable across multiple fields, including immunology, cancer research, and infectious disease.
How does solid-phase phage display differ from solution-phase screening
In solid-phase screening, the target is immobilized on a surface, whereas, in solution-phase screening, the target remains in a liquid medium. Solid-phase screening allows for easier separation of bound and unbound phages, reducing background noise and enabling more efficient identification of high-affinity binders. Solution-phase screening, on the other hand, may offer more natural binding conditions, but with increased complexity in washing and separation steps.
What are the typical steps involved in solid-phase phage display screening
The typical steps include immobilizing the target on a solid surface, incubating the phage display library with the target, washing away unbound phages, and eluting bound phages. The bound phages are then amplified and subjected to additional rounds of selection to enrich for high-affinity binders. After multiple rounds, the DNA of the selected phages is sequenced to identify the binding peptides.
How is solid-phase phage display used in antibody discovery
Solid-phase phage display is a critical technique in antibody discovery, enabling the identification of antibodies that bind to specific antigens immobilized on a surface. By screening large antibody libraries, this method allows for the selection of high-affinity and specific antibodies, which can then be further developed as diagnostic tools, therapeutic agents, or research reagents.