Advancing induced pluripotent stem cell (iPSC) therapy research to clinical applications requires careful material selection because the quality of starting materials significantly impacts the properties of your final stem cell therapy product. iPSCs are known to secrete numerous components that possess important biological functions. These components usually contain bioactive factors derived from iPSC but the formulated product does not contain any stem cells. They could be used for further analysis in safety and efficacy in preclinical models.
Cells could be activated, expanded, and differentiated with the help of cytokines, chemokines, as well as growth factors derived from iPSC culture process. These high-quality reagents ensure reproducibility across a variety of applications, including immunology and stem cell research. For instance, in cardiovascular disease research, iPSC can secrete a wide array of cytokines and growth factors for cardiac repair via modulation of the inflammatory response, inhibition of cell necrosis and apoptosis, and promotion of angiogenesis. Besides, cytokines/chemokines/growth factors cocktails have also emerged to enhance the rejuvenation of aging or damaged cell.
A conditioned medium generated by different types of stem cells under specific culture conditions has been shown to improve various types of cell culture conditions. It enhances the proliferation of stem cells in a state of non-conversion and provides a high proliferation ability and uniform cell state at culturing. At Creative Biolabs, the medium is generated from a novel pooling process of the cells and conditioned medium, reducing batch to batch variability. Our patented culture process yields a stable secretion of components in specified amounts.
Neurotrophic factors (NTFs) are endogenous survival factors for developing neurons during their programmed cell death and represent therapeutic candidates in several neurodegenerative diseases. NTFs have been shown to rescue retinal neurons in different models of retinal disease.
iPSCs are characterized by their ability to self-renew and iPSCs differentiate into any cell type. The molecular mechanism behind this process is a complex interplay between the transcriptional factors with epigenetic regulators and signaling pathways. microRNAs are an integral part of this regulatory network with essential roles in pluripotent maintenance, proliferation and differentiation. Several miRNA clusters, such as miR-181a, miR-199b-3p and miR-214 are human iPSC-specific miRNAs that have an important role in maintaining pluripotency and cellular reprogramming. miR-290-295 and miR-302-367 can dramatically promote reprogramming into iPSCs and, in the case of miR-302-367, can even completely substitute for the standard transcription factors OCT4, SOX2, KLF4 and MYC.
For Research Use Only. Not For Clinical Use.
