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In vitro assays for stem cell research involve the manipulation and evaluation of stem cells in a controlled laboratory setting outside of an organism's body. These assays are crucial for understanding the behavior, differentiation potential, and therapeutic applications of stem cells.
These assays typically involve culturing stem cells in vitro, exposing them to specific growth factors or conditions, and monitoring their behavior and characteristics. Researchers may use a variety of techniques such as immunocytochemistry, flow cytometry, gene expression analysis, and functional assays to assess stem cells' properties and responses. These assays continue to play a crucial role in driving cutting-edge research in the field of stem cell biology and regenerative medicine.
Our team of experienced scientists utilizes state-of-the-art technologies and methodologies to conduct a variety of in vitro assays tailored to meet the specific needs of our clients. Some of the key in vitro assay services we offer include:
The appropriate inducer and induction mode are selected to differentiate the stem cells in a predetermined cell type to obtain the cell type desired by the human.
The desired cells are separated from the body in a sterile environment, cultured under appropriate conditions, and tested for cell proliferation ability and cell cycle.
After the cells reached a suitable density, the proliferation of the cells was examined by the MTT method and the CCK8 method.
Cell cycle and apoptosis were determined by flow cytometry in conjunction with PI staining.
It mainly studies the effects of chemokines or other nutrients on cell invasion.
Cell migration is one of the basic functions of normal cells, and many physiological processes in the body involve cell migration. Scratch experiments and Transwell can be used to detect the ability of cells to migrate.
If you need professional, reliable and quality services, please entrust our professional team to provide the best workflow choice for your research goals. Our wide range of service capabilities, consistently exporting high quality, consistent products, has been highly recognized by the industry. With the help of our well-established technology and experienced scientists, Creative Biolabs provides comprehensive in vitro assay services for customers. For more information, please feel free to contact us.
Below are the findings presented in the article related to the in vitro assay for stem cells.
Stem cell transplantation has emerged as an alternative method for bone repair and has attracted much attention due to its excellent bioactivity and therapeutic efficacy. Lei Yang et al. developed a novel core-shell microcapsule containing stem cells in the core and biomass in the shell by using all-water phase microfluidic electrospray technology. This study demonstrated that core-shell microcapsules containing stem cells have good biocompatibility and therapeutic effects on bone defects.
Osteoblasts are the main somatic cells of bone tissue, and BMSCs are the main stem cells of bone tissue. Therefore, they performed in vitro osteoblast and stem cell biocompatibility tests with a pre-osteoblast cell line and primary stem cell BMSC. These data indicate that hydrogel microcapsules have good biocompatibility in vitro and that the microcapsules can support in vitro growth and osteogenic differentiation of BMSC.
Fig. 1 Cell proliferation and biocompatibility test in vitro.1
Reference
For Research Use Only. Not For Clinical Use.