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In Vitro Assay Development Service

Overview Service Features Published Data FAQs Scientific Resources Related Services

With the leading technology and years of experience in biomedical science, Creative Biolabs offers a full range of assay development services. As a leading service provider, our goal is to provide the highest level of service, confidentiality and customer support. Our in vitro assay development service will promote the development of your project.

Overview of In Vitro Assays for Stem Cell Research

In vitro assays for stem cell research involve the manipulation and evaluation of stem cells in a controlled laboratory setting outside of an organism's body. These assays are crucial for understanding the behavior, differentiation potential, and therapeutic applications of stem cells.

These assays typically involve culturing stem cells in vitro, exposing them to specific growth factors or conditions, and monitoring their behavior and characteristics. Researchers may use a variety of techniques such as immunocytochemistry, flow cytometry, gene expression analysis, and functional assays to assess stem cells' properties and responses. These assays continue to play a crucial role in driving cutting-edge research in the field of stem cell biology and regenerative medicine.

Background2

In vitro Assay Services

Our team of experienced scientists utilizes state-of-the-art technologies and methodologies to conduct a variety of in vitro assays tailored to meet the specific needs of our clients. Some of the key in vitro assay services we offer include:

Cell Growth Assay

  • Stem cell differentiation

    The appropriate inducer and induction mode are selected to differentiate the stem cells in a predetermined cell type to obtain the cell type desired by the human.

  • Primary cell separation

    The desired cells are separated from the body in a sterile environment, cultured under appropriate conditions, and tested for cell proliferation ability and cell cycle.

  • Cell proliferation assay

    After the cells reached a suitable density, the proliferation of the cells was examined by the MTT method and the CCK8 method.

  • Cell cycle and apoptosis detection

    Cell cycle and apoptosis were determined by flow cytometry in conjunction with PI staining.

  • Cell invasion

    It mainly studies the effects of chemokines or other nutrients on cell invasion.

  • Cell migration

    Cell migration is one of the basic functions of normal cells, and many physiological processes in the body involve cell migration. Scratch experiments and Transwell can be used to detect the ability of cells to migrate.

Other Assays

  • Senescence assays
  • Co-culture
  • Autophagy assays

Features of Our Services

  • Highly efficient
  • Skillful scientific team
  • Short time and low cost
  • All-round comprehensive service

If you need professional, reliable and quality services, please entrust our professional team to provide the best workflow choice for your research goals. Our wide range of service capabilities, consistently exporting high quality, consistent products, has been highly recognized by the industry. With the help of our well-established technology and experienced scientists, Creative Biolabs provides comprehensive in vitro assay services for customers. For more information, please feel free to contact us.

Published Data

Below are the findings presented in the article related to the in vitro assay for stem cells.

Stem cell transplantation has emerged as an alternative method for bone repair and has attracted much attention due to its excellent bioactivity and therapeutic efficacy. Lei Yang et al. developed a novel core-shell microcapsule containing stem cells in the core and biomass in the shell by using all-water phase microfluidic electrospray technology. This study demonstrated that core-shell microcapsules containing stem cells have good biocompatibility and therapeutic effects on bone defects.

Osteoblasts are the main somatic cells of bone tissue, and BMSCs are the main stem cells of bone tissue. Therefore, they performed in vitro osteoblast and stem cell biocompatibility tests with a pre-osteoblast cell line and primary stem cell BMSC. These data indicate that hydrogel microcapsules have good biocompatibility in vitro and that the microcapsules can support in vitro growth and osteogenic differentiation of BMSC.

Cell proliferation and biocompatibility test in vitro. (Yang, Lei, et al., 2022)Fig. 1 Cell proliferation and biocompatibility test in vitro.1

FAQs

  • Q: Can you customize the assays based on specific research requirements, such as drug screening or toxicity testing?
    A: Absolutely! Our in vitro assay development service is fully customizable to meet your specific research needs. Whether you require assays for drug screening, toxicity testing, or differentiation efficiency, we can tailor the assays accordingly. We work closely with you to understand your objectives and design assays that provide the most relevant data. Our team has extensive experience in developing bespoke assays that align with your unique research goals.
  • Q: How do you handle the scalability of the assays for large-scale screening or high-throughput applications?
    A: Scalability is a key consideration in our assay development process. We design assays that can be easily adapted for large-scale screening and high-throughput applications. Our platforms are compatible with various formats, including 96-well and 384-well plates, ensuring seamless transition from small-scale validation to large-scale screening. We also utilize automation and advanced imaging technologies to handle high-throughput demands efficiently, delivering consistent results across large datasets.
  • Q: What level of support do you offer throughout the assay development process?
    A: We provide comprehensive support from project initiation to final data delivery. Our team collaborates with you during the initial consultation to define assay objectives and design the experimental setup. Throughout the project, you will receive regular updates, data analysis, and recommendations for optimizing the assays. Post-development, we offer troubleshooting assistance, data interpretation guidance, and follow-up consultations to ensure the assays meet your long-term research needs.
  • Q: Can you integrate multiple readouts into a single assay?
    A: Absolutely! We specialize in multiplex assays that combine various readouts, such as cell viability, differentiation status, and gene expression levels. By integrating multiple endpoints into a single assay, we can provide a more comprehensive view of your stem cell model. Our team is skilled in balancing assay sensitivity, accuracy, and robustness, ensuring that all relevant data points are captured in a single workflow.

Scientific Resources

Reference

  1. Yang, Lei, et al. "Biomass microcapsules with stem cell encapsulation for bone repair." Nano-Micro Letters 14 (2022): 1-12.

For Research Use Only. Not For Clinical Use.