E. coli Lysate based Cell-free Membrane Protein Expression Service

As an easy-to-use membrane protein expression system, Magic™ cell-free membrane protein expression in E. coli lysate is used for in vitro cell-free membrane protein synthesis in a single-tube reaction. The kit utilizes an efficient, coupled transcription/translation reaction to express and manufacture the soluble, functionally active membrane protein in as little as 3-6 hours. The in vitro synthesis reactions can be easily miniaturized or scaled up to yield milligram quantities of the target membrane protein. Moreover, the target membrane proteins can be used in various downstream applications.

Overview

Produce active membrane proteins in a single-tube reaction.
Eliminates the time-consuming procedures of cell-based membrane protein expression system.
Easy-to-use system allows generation of target membrane protein in just 3-6 hours.
DNA template can be circular plasmid or linear PCR products.
Allows you to perform high-throughput synthesis of membrane proteins.
Expression of toxic proteins that are lethal for in vivo expression systems.
Suitable for a variety of downstream applications and functional studies.

Kit Description

Magic™ Cell-free Membrane Protein Expression in E. coli Lysate Kit can be used for in vitro membrane protein synthesis at high levels. It is noticed that this kit can synthesize membrane proteins from both circular plasmid and linear templates (e.g. PCR products). In terms of the template, it should contain a T7 promoter. The initial protein synthesis reaction is composed of E. coli extract, reaction buffer, and amino acids. After initiating the protein synthesis reaction, supplemental buffer is added in the reaction to replenish the necessary components which are depleted or degraded during protein synthesis. Supplement with the buffer allows continuous cell-free membrane protein synthesis, resulting in membrane protein production in a higher yield in up to 3-6 hours. We recommend wearing gloves and using nuclease-free tubes and tips to avoid introducing nucleases to your samples.

Components List and Storage Information

Within the kit, it contains optimized all necessary components, listed in the table below. Simply mix these reagents with the target template and incubate them at 30-37 °C in few hours. Subsequently, analyze or purify the proteins (if needed) or store the proteins at -20°C for further processing or analysis. The kit can be used for high throughput screening, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions.

Expression System	Components	Storage
In Vitro E. coli Expression System	E. coli Extract	-80°C
	2.5 × Reaction Buffer	-80°C
	2 × Supplemental Buffer	-80°C
	T7 Enzyme Mix	-20°C
	Amino Acid Mix (No Met)	-20°C
	Methionine	-20°C
	DNase/RNase-Free Water	RT
	Control Vector	-20°C

Features

Facilitate the production of target membrane proteins in high levels
Simple protocol and easy setup with all necessary components
The target membrane proteins can be used in various downstream applications
Membrane protein of interest can be synthesized and visualized in a few hours
Low cost

Applications

Expression of membrane proteins that are easily degraded or insoluble in conventional cell-based expression systems
Full-length, functional membrane protein expression
Toxic or difficult to express membrane protein synthesis
High-throughput screening
Synthetic biology
Membrane protein folding studies
Rapid analysis of membrane protein mutations or truncations
Membrane protein activity and protein-protein interactions

Creative Biolabs is a world-leading product provider for various cell-free membrane protein expression systems. Moreover, we now provide integrated Magic™ membrane protein expression services in various cell-free expression systems for our clients around the world. To get more information, please feel free to contact us.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-1	Magic™ Cell-free Membrane Protein Expression in E. coli Lysate	0.75 mg/mL
MPCFK-1-1	Magic™ Simplified Cell-free Membrane Protein Expression in E. coli Lysate	0.45 mg/mL
MPCFK-1-2	Magic™ Cell-free Membrane Protein Expression in E. coli Lysate - Dialysis Mode	5-10 mg/mL

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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