As an easy-to-use membrane protein expression system, Magic™ cell-free membrane protein expression in E. coli lysate is used for in vitro cell-free membrane protein synthesis in a single-tube reaction. The kit utilizes an efficient, coupled transcription/translation reaction to express and manufacture the soluble, functionally active membrane protein in as little as 3-6 hours. The in vitro synthesis reactions can be easily miniaturized or scaled up to yield milligram quantities of the target membrane protein. Moreover, the target membrane proteins can be used in various downstream applications.
Magic™ Cell-free Membrane Protein Expression in E. coli Lysate Kit can be used for in vitro membrane protein synthesis at high levels. It is noticed that this kit can synthesize membrane proteins from both circular plasmid and linear templates (e.g. PCR products). In terms of the template, it should contain a T7 promoter. The initial protein synthesis reaction is composed of E. coli extract, reaction buffer, and amino acids. After initiating the protein synthesis reaction, supplemental buffer is added in the reaction to replenish the necessary components which are depleted or degraded during protein synthesis. Supplement with the buffer allows continuous cell-free membrane protein synthesis, resulting in membrane protein production in a higher yield in up to 3-6 hours. We recommend wearing gloves and using nuclease-free tubes and tips to avoid introducing nucleases to your samples.
Within the kit, it contains optimized all necessary components, listed in the table below. Simply mix these reagents with the target template and incubate them at 30-37 °C in few hours. Subsequently, analyze or purify the proteins (if needed) or store the proteins at -20°C for further processing or analysis. The kit can be used for high throughput screening, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions.
Expression System | Components | Storage |
In Vitro E. coli Expression System | E. coli Extract | -80°C |
2.5 × Reaction Buffer | -80°C | |
2 × Supplemental Buffer | -80°C | |
T7 Enzyme Mix | -20°C | |
Amino Acid Mix (No Met) | -20°C | |
Methionine | -20°C | |
DNase/RNase-Free Water | RT | |
Control Vector | -20°C |
Creative Biolabs is a world-leading product provider for various cell-free membrane protein expression systems. Moreover, we now provide integrated Magic™ membrane protein expression services in various cell-free expression systems for our clients around the world. To get more information, please feel free to contact us.
Cat | Product Name | Expected Yield of Positive Control |
MPCFK-1 | Magic™ Cell-free Membrane Protein Expression in E. coli Lysate | 0.75 mg/mL |
MPCFK-1-1 | Magic™ Simplified Cell-free Membrane Protein Expression in E. coli Lysate | 0.45 mg/mL |
MPCFK-1-2 | Magic™ Cell-free Membrane Protein Expression in E. coli Lysate - Dialysis Mode | 5-10 mg/mL |
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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