Wheat Germ Lysate based Cell-free Membrane Protein Expression Service

Magic™ cell-free membrane protein expression in wheat germ lysate is a cell-free system using wheat germ extracts for membrane protein production. By adding liposomes, artificially-prepared spherical lipid vesicles, to the translation reaction, membrane proteins are directly inserted into the lipid bilayer of the liposome to form proteoliposome complexes. In Magic™ Cell-free Membrane Protein Expression in Wheat Germ Lysate Kit, protein expression reactions can easily be set up with few pipetting steps, and no fresh liposomes have to be prepared for each experiment.

Which proteins can be expressed in wheat germ cell-free system?

CFMPE system from wheat germ extract can be utilized in the expression of both prokaryotic and eukaryotic proteins, which provides the best yield among the eukaryotic systems. In particular, this system is suitable for the production of proteins with complicated conformation and multiple protein complexes, such as disulfide-bond-containing or integral membrane proteins. It is noticed that this system does not require codon optimization even for the expression of transcripts with a high A/T or G/C content.

Components List and Storage

Expression System	Components	Storage
In Vitro Wheat Germ Expression System	Wheat Germ Extracts	-80°C
	Reaction buffer	-80°C
	Transcription Reagent	-80°C
	Aluminum seal	RT
	pEU-E01-MCS	-80°C
	pEU-E01-T1R1 (Positive Control)	-80°C

Advantages

Membrane protein has been synthesized using proteoliposome
No need to prepare reagents
Reagents necessary for each reaction are already pre-packaged in tubes
Synthesize proteins with just a few pipetting steps
Target membrane proteins can be confirmed through SDS-PAGE/CBB staining
Contains enough for 8 reactions on a 240 µL reaction scale

Applications

Expression screening of membrane protein
Quick analysis of protein functionality, mutations, or truncations
Structural genomics, isotope labeling, or incorporating unnatural amino acid and crystallography
High-throughput proteomic studies

CFMPE is the production of membrane proteins in solution using biomolecular translation machinery extracted from cells of different species. Creative Biolabs now provides the most advanced wheat germ cell-free system for membrane protein expression. We have developed a series of cell-free expression systems for high-quality, large/medium-scale membrane protein expression. Equipped with state-of-the-art technology platforms and extensive expertise, our one-stop integrated services can help our worldwide clients to boost their diverse research and projects. To discuss your plans for membrane protein expression, please feel free to contact us for more details.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-3	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate with liposome	40 µg/mL
MPCFK-3-1	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate	0.1-0.2 mg/mL
MPCFK-3-2	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate for protein expression screening	20 µg/mL
MPCFK-3-3	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate - Bilayer Format	20-40 µg/mL
MPCFK-3-4	Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for Protein Production	20-40 µg/mL
MPCFK-3-5	Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for GST-tagged Protein Production	20-40 µg/mL
MPCFK-3-6	Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Production	20-40 µg/mL
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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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