Magic™ cell-free membrane protein expression in wheat germ lysate is a cell-free system using wheat germ extracts for membrane protein production. By adding liposomes, artificially-prepared spherical lipid vesicles, to the translation reaction, membrane proteins are directly inserted into the lipid bilayer of the liposome to form proteoliposome complexes. In Magic™ Cell-free Membrane Protein Expression in Wheat Germ Lysate Kit, protein expression reactions can easily be set up with few pipetting steps, and no fresh liposomes have to be prepared for each experiment.
CFMPE system from wheat germ extract can be utilized in the expression of both prokaryotic and eukaryotic proteins, which provides the best yield among the eukaryotic systems. In particular, this system is suitable for the production of proteins with complicated conformation and multiple protein complexes, such as disulfide-bond-containing or integral membrane proteins. It is noticed that this system does not require codon optimization even for the expression of transcripts with a high A/T or G/C content.
Expression System | Components | Storage |
In Vitro Wheat Germ Expression System | Wheat Germ Extracts | -80°C |
Reaction buffer | -80°C | |
Transcription Reagent | -80°C | |
Aluminum seal | RT | |
pEU-E01-MCS | -80°C | |
pEU-E01-T1R1 (Positive Control) | -80°C |
CFMPE is the production of membrane proteins in solution using biomolecular translation machinery extracted from cells of different species. Creative Biolabs now provides the most advanced wheat germ cell-free system for membrane protein expression. We have developed a series of cell-free expression systems for high-quality, large/medium-scale membrane protein expression. Equipped with state-of-the-art technology platforms and extensive expertise, our one-stop integrated services can help our worldwide clients to boost their diverse research and projects. To discuss your plans for membrane protein expression, please feel free to contact us for more details.
Cat | Product Name | Expected Yield of Positive Control |
MPCFK-3 | Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate with liposome | 40 µg/mL |
MPCFK-3-1 | Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate | 0.1-0.2 mg/mL |
MPCFK-3-2 | Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate for protein expression screening | 20 µg/mL |
MPCFK-3-3 | Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate - Bilayer Format | 20-40 µg/mL |
MPCFK-3-4 | Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for Protein Production | 20-40 µg/mL |
MPCFK-3-5 | Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for GST-tagged Protein Production | 20-40 µg/mL |
MPCFK-3-6 | Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Production | 20-40 µg/mL |
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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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