Rabbit Anti-Idiotypic Antibody Discovery Service

Overview of Our Services Types of Rabbit Anti-ID Antibody Advantages of Rabbit Anti-ID Antibody Applications Service Highlights Published Data

Overview of Our Services

Creative Biolabs offers rabbit anti-idiotypic antibody production service. We have established a world-leading platform for rabbit anti-idiotypic antibody selection, production, and engineering. Our scientists have developed high-quality rabbit anti-idiotypic antibodies against several therapeutic antibodies. We offer the development of polyclonal and monoclonal rabbit anti-idiotypic antibodies. Polyclonal antibodies from rabbits are suitable if you need a lower amount of antibodies within a short time, e. g. for pre-studies. If the antibodies shall be used in long-term studies under validated conditions, then monoclonal antibodies are preferable.

As rabbit antibodies often carry multiple glycans on the heavy chain Fc part (which cannot be removed by PNgase F), intact mass determination is a general problem for rabbit antibodies (with or without BSA). However, our sequences are of such high quality that validation by intact mass is typically not required. Also please keep in mind that rabbit antibodies have an additional SH-bridge from the VL variable region to the VC region. This SH-bridge does not exist in mouse, hamster, rat and human antibodies.

Types of Rabbit Anti-ID Antibody

Based on binding modes and characteristics, rabbit anti-idiotypic antibodies are categorized into antigen non-blocking type, antigen blocking type, and drug target complex types.

Antigen Non-blocking Type lack specificity for the antibody binding region, allowing therapeutic antibodies to continue binding with target antigens, making them suitable for detecting total antibody drugs.
Antigen Blocking Type possess specificity for the antibody binding region, competing with target antigens, and are thus used to detect free antibody drugs.
Drug-target Complex Specific anti-idiotypic antibodies specifically recognize antibody-target complexes, without binding to unbound antibodies or antigens, enabling the detection of only bound antibody drugs.

Advantages of Rabbit Anti-ID Antibody

High specificity: Rabbit monoclonal antibodies exhibit higher specificity compared to mouse monoclonal antibodies due to a dual screening mechanism in the rabbit immune system including positive-feedback and negative-feedback, resulting in less non-specific interference.
High diversity: The rabbit immune system allows for the production of a diverse array of antibodies, with a higher capacity to generate both inhibitory and non-inhibitory anti-idiotypic antibodies.
More epitopes can be recognized: Rabbits can produce antibodies against a broader range of antigens, recognizing epitopes that are often missed by mouse antibodies, especially in the case of small molecule antigens or post-translational modification targets.
High affinity: Rabbit monoclonal antibodies have an affinity 10-100 times greater than that of mouse monoclonal antibodies, leading to enhanced sensitivity in detecting monoclonal antibody drugs in biological samples.
Higher manufacturing efficiency: The larger spleen and greater B cell content in rabbits enable the production of a higher quantity of antibodies at once, improving the efficiency of monoclonal antibody production through high-throughput screening.

Applications

Immunogenicity Studies	Pharmacokinetic (PK) Analysis
Anti-idiotypic antibodies are employed as positive controls in the detection of anti-drug antibodies (ADA) to simulate real blood sample scenarios due to their specificity and cost-effectiveness, enhancing the assessment of the immune response towards biopharmaceuticals.	Anti-idiotypic antibodies serve as crucial reagents for PK analysis in both preclinical and clinical settings, allowing for the precise evaluation of drug dosage and toxicity by detecting free or total drug levels in serum.

Service Highlights

• Fast delivery
• Competitive price
• Modification of antibody as required
• High specificity and binding affinity

Published Data

Anti-idiotypic antibodies targeting antibodies against retinol-binding protein (RBP) and insulin have demonstrated specificity for the antigen-binding sites of their respective immunogens. Some rabbit anti-idiotypic antibodies against RBP antibodies bind selectively to rat intestinal epithelial cells, which house a cell-surface receptor for RBP facilitating retinol uptake. Remarkably, these antibodies impeded this uptake by competing with RBP. Similarly, antibodies against insulin antibodies hindered the binding of iodine-125 labeled insulin to rat fat cells and simulated insulin's action by encouraging α-aminoisobutyric acid uptake in rat thymocytes. Such findings highlight anti-idiotypic antibodies as potential instruments for exploring cell-membrane receptor functionalities.

Creative Biolabs specializes in the tailored development of various anti-idiotypic antibodies to complement your therapeutic antibodies. Click here to explore our bespoke Anti-Idiotypic Antibodies Production Services designed to cater to your specific needs.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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