What is a Fab fragment in the context of this ADC?

A Fab fragment represents one of the arms of an antibody, consisting of one constant and one variable domain from both the heavy and light chains, providing antigen binding capability without the entire antibody structure.

Why use a cleavable linker in this ADC?

A cleavable linker allows the cytotoxic drug MMAF to be released upon entering the target cell, enhancing the drug's effectiveness and reducing systemic toxicity.

How does the cleavable linker function?

The linker is designed to be stable in the bloodstream but breaks down in the acidic environment of the cell's lysosomes or in the presence of certain cellular enzymes, releasing the MMAF to exert its cytotoxic effects.

What are the challenges in developing Fab fragment-based ADCs?

Ensuring that the Fab fragment retains its binding efficiency and specificity after conjugation with the cytotoxic drug is a significant challenge.

How does the ADC manage to show minimal toxicity without a primary antibody?

The specificity of the Fab fragment for the Fc region and the stability of the cleavable linker ensure that MMAF is activated primarily in the presence of target antigens, reducing off-target effects.

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anti-HIgG(Fc)Fab-C-MMAF ADC

anti-HIgG(Fc)Fab-C-MMAF ADC (CAT#: ADC-AA-010)

This ADC product is comprised of a Fab fragment of an anti-human IgG Fc specific polyclonal antibody conjugated via a cleavable linker to MMAF. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening human monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Fab fragment of anti-human IgG Fc specific polyclonal antibody

Species Reactivity
Human

Name
Cleavable linkers

Description
Cleavable linkers rely on the physiological stimuli, which mainly include chemically cleavable linkers and enzymatically cleavable linkers. Chemically cleavable linkers including acid-labile linkers and disulfide linkers. For acid-labile linkers, intracellular release of payloads relies on the different pH between endosomes/lysosomes and blood. The release of disulﬁde-linked drugs is controlled by the factors in intracellular environment. Enzymatically cleavable linkers, peptide linkers and β-glucuronide linkers, are sensitive to enzymes located in cytoplasm.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The anti-HIgG(Fc)Fab-C-MMAF ADC from Creative Biolabs has greatly improved our pre-screening process for human monoclonal antibody candidates. The linkage to MMAF allows precise targeting, and we've observed no significant toxicity in absence of a primary antibody. Highly recommend for efficient and economical screening.

Excellent

I've used Creative Biolabs' anti-HIgG(Fc)Fab-C-MMAF ADC in several experiments, and I'm impressed by its efficacy in inducing cell death through microtubule disruption. The product is reliable and significantly accelerates our antibody development pipeline.

Excellent

Our lab switched to using the anti-HIgG(Fc)Fab-C-MMAF ADC for preliminary ADC screening, and we couldn't be happier. The Fab fragment's specificity combined with MMAF's potent cytotoxicity has yielded excellent results without the toxicity concerns typical of full-length antibody conjugates.

Excellent

Fantastic product

The cleavable linker in the anti-HIgG(Fc)Fab-C-MMAF ADC offers a controlled release of MMAF, making it an invaluable tool for our studies on tubulin dynamics and cell cycle arrest. We've noted a marked improvement in our testing throughput.

Excellent

As a frequent user of Creative Biolabs' products, the anti-HIgG(Fc)Fab-C-MMAF ADC stands out for its effectiveness and safety. It's been a game changer in our efforts to identify promising ADC candidates more rapidly and cost-effectively.

Excellent

The precision of the anti-HIgG(Fc)Fab-C-MMAF ADC in targeting and inducing cytotoxic effects is remarkable. It's helped us refine our selection of antibodies for further development. An essential addition to any biotech lab working with ADCs.

Q: 1: What distinguishes Anti-HIgG(Fc)Fab-C-MMAF ADC from other ADCs?
A: This ADC is distinguished by its use of a Fab fragment of an anti-human IgG Fc specific polyclonal antibody, conjugated via a cleavable linker to MMAF, which allows for a targeted and controlled release of the cytotoxic drug.
Q: 2: What is a Fab fragment in the context of this ADC?
A: A Fab fragment represents one of the arms of an antibody, consisting of one constant and one variable domain from both the heavy and light chains, providing antigen binding capability without the entire antibody structure.
Q: 3: Why use a cleavable linker in this ADC?
A: A cleavable linker allows the cytotoxic drug MMAF to be released upon entering the target cell, enhancing the drug's effectiveness and reducing systemic toxicity.
Q: 4: How does the cleavable linker function?
A: The linker is designed to be stable in the bloodstream but breaks down in the acidic environment of the cell's lysosomes or in the presence of certain cellular enzymes, releasing the MMAF to exert its cytotoxic effects.
Q: 5: What are the challenges in developing Fab fragment-based ADCs?
A: Ensuring that the Fab fragment retains its binding efficiency and specificity after conjugation with the cytotoxic drug is a significant challenge.
Q: 6: How does the ADC manage to show minimal toxicity without a primary antibody?
A: The specificity of the Fab fragment for the Fc region and the stability of the cleavable linker ensure that MMAF is activated primarily in the presence of target antigens, reducing off-target effects.

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Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-021	anti-MIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-054	Protein A-MCC-DM1 ADC	MCC (Maleimidomethyl cyclohexane-1-carboxylate)	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-026	anti-MIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-055	Protein A-Duocarmycin ADC		Duocarmycins

CAT#	Product Name	Linker	Payload
WJY-0423-LS106	Protein A-Calicheamicin ADC	Cleavable linkers	Calicheamicin
ADC-AA-028	anti-MIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-003	anti-HIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-020	anti-MIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-021	anti-MIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)

CAT#	Product Name	Linker	Payload
ADC-W-515	Anti-EGFR (ABT-806)-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-W-485	Anti-EphA2 (clone 1C1)-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-028	anti-MIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-491	Anti-TNFRSF17-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)

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