anti-MIgG(Fc)-C-MMAF ADC (CAT#: ADC-AA-020)

A: The anti-MIgG(Fc)-C-MMAF ADC consists of an anti-mouse IgG Fc specific polyclonal antibody conjugated to the cytotoxic small molecule MMAF via a cleavable linker. The antibody serves as a secondary antibody, while MMAF targets tubulins, disrupting microtubule dynamics and inducing cell death.

A: This ADC is primarily used for pre-screening mouse monoclonal antibodies as ADC candidates. It is an efficient and economical alternative for this purpose, especially since it displays no obvious toxicity without a primary antibody.

A: The ADC works by the MMAF portion binding to tubulins within the cell, interrupting microtubule dynamics and ultimately leading to cell death. The antibody portion targets the Fc region of mouse IgG, facilitating the delivery of MMAF to the intended site.

A: The Fc region of IgG is crucial because it contains a highly conserved N-glycosylation site necessary for Fc receptor-mediated activity. This region binds to various cell receptors and complement proteins, mediating several physiological effects such as opsonization and antibody-dependent cellular cytotoxicity (ADCC).

A: It is recommended to store the ADC at -80°C and protect it from light. Proper handling procedures should be followed to ensure the stability and efficacy of the product. Always refer to the product datasheet for detailed storage and handling instructions.