CLCA3P Membrane Protein Introduction

Introduction of CLCA3P

CLCA3P, also known as CLCA3, is a protein encoded by the CLCA3 pseudogene. The protein is a chloride channel which is not only expressed in human but also in certain other species such as mouse. CLCA3 has been identified in goblet cells throughout the intestinal tract by in situ hybridization. The CLCA3 transcript is also detected in the murine trachea and uterus. However, the function and regulation of CLCA3 are still not very clear. The biologic processes involved are also unclear.

Function of CLCA3P Membrane Protein

The calcium-activated chloride channels (CLCA) family appears to mediate a calcium-activated chloride conductance in a variety of tissues, including epithelium, skeletal muscle and neurons. Six members of this family have been identified, cloned and partially characterized in the mouse: Clca1, Clca2, Clca3, Clca4, Clca5, and Clca6. CLCA3 has been identified in goblet cells throughout the intestinal tract by in situ hybridization. This protein always takes part in molecular function, such as calcium channel activity, chloride channel activity, metalloendopeptidase activity, transporter activity and voltage-gated ion channel activity. Its function as ion channel gives itself a role in ion transmembrane transport and regulation of ion transmembrane transport. Activation of the CLCA3 may also participate in sensation processes such as pain, warmth, cold, taste pressure and vision through stimuli-sensing channels.

Application of CLCA3P Membrane Protein in Literature

1. Li W., et al. P. aeruginosa lipopolysaccharide-induced MUC5AC and CLCA3 expression is partly through Duox1 in vitro and in vivo. PLoS One. 2013, 8(5): e63945. PubMed ID: 23691121
   
   

   The authors examine PA-LPS-induced Duox1 mRNA levels in A549 cells, primary mouse tracheal epithelial cells (mTECS) and lung tissues of mice. They then use Nox inhibitors diphenyleneiodonium chloride (DPI) and Duox1 siRNA both in vitro and in vivo. The results demonstrate that MUC5AC and CLCA3 expression induced by PA-LPS is partly companied with Duox1 and provide supportive evidence for Duox1 as a potential target in treatments of mucin over-production diseases at the same time.

2. Jeong J.W., et al. Steroid hormone regulation of CLCA3 expression in the murine uterus. J Endocrinol. 2006, 189(3):473-84. PubMed ID: 16731779
   
   

   In this study, authors identify a subset of genes whose expression is repressed by chronic P4-PGR activation in the uterus by using high-density DNA microarray analysis. The CLCA3 gene is also one of the genes whose expression is negatively related to P4 and PGR. Their study finally identifies CLCA3 as a novel downregulated gene of PGR, which is a direct target of E2 regulation.

3. Morton J.D., et al. Functional genomics of asthma: Role of CLCA3 in goblet cell metaplasia (GCM) but not airway hyperreactivity (AHR). Journal of Allergy and Clinical Immunology. 2004, 113(2): S205.
   
   

   This article indicates that CLCA3 gene expression is selectively associated with GCM. Furthermore, expression of CLCA3 can bring about MUC5AC-positive goblet cell formation both ex vivo (in cultured mouse tracheal epithelial cells) and in vivo. In conclusion, CLCA3 expression appears to be a selective determinant of GCM and thus may represent a useful target for focused therapeutic intervention in hypersecretory states such as asthma.

4. Dietert K., et al. mCLCA3 modulates IL-17 and CXCL-1 induction and leukocyte recruitment in murine staphylococcus aureus pneumonia. PLOS ONE. 2014, 9(7):e102606. PubMed ID: 25033194
   
   

   The experimental data suggest that mCLCA3 may be involved in the modulation leukocyte response via IL-17 and murine CXCL-8 homologs in acute Staphylococcus aureus pneumonia which is well similar with the proposed function of hCLCA1 as a signaling molecule acting on alveolar macrophages.

5. Mundhenk L., et al. mCLCA3 does not contribute to calcium-activated chloride conductance in murine airways. American Journal of Respiratory Cell and Molecular Biology. 2012, 47(1):87-93. PubMed ID: 22362387
   
   

   The findings in the study shows that mCLCA3 may not involves the CaCC-mediated Cl(-) secretion in murine respiratory epithelia.

CLCA3P Preparation Options

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