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With years of experience in complement tests, Creative Biolabs supports complement C5 functional test to help advance your programs. Notably, Creative Biolabs can tailor complement test assays based on multi-disciplinary scientists and comprehensive platforms.
Overview of Complement C5
The complement component C5 is part of the innate immune system. The complement system consists of a group of proteins that work together to defend against foreign pathogens, promote inflammation, and facilitate the clearance of damaged cells or immune complexes. The C5 protein is a critical component of the complement cascade. When activated, it plays a role in the formation of the membrane attack complex (MAC), which can lyse target cells or pathogens.
Introduction of Complement C5 Functional Test
Method
Hemolytic assays are one of the primary complement C5 function tests that may be performed. By combining patient serum with a C5-deficient serum, component C5 activity is assessed. The serum mixture's lytic activity is evaluated against SRBC or sensitized, tagged liposomes. If lysis happens, the C5 must originate from the patient's serum. In addition, we offer hemolytic assays with inhibitors or C5 mutant proteins.
Analysis
Low levels of C5 may indicate a deficiency that could impact the overall functionality of the complement system. The complement C5 functional test is typically performed in specialized laboratories and is often used in the diagnosis and monitoring of complement deficiencies, autoimmune disorders, and certain infections.
Fig. 1 Function of complement C5.1, 4
Workflow of Complement C5 Functional Test
Hemolytic assays
Fig. 2 An example of C5 result with hemolytic assays.2, 4
C5 Elisa
Case Study
Fig. 3 C5a was estimated by a specific ELISA.3, 4
Applications
Diagnosis of Complement Deficiencies
Deficiencies in complement components, including C5, can lead to an increased susceptibility to certain infections, autoimmune diseases, or inflammatory conditions. The complement C5 functional test can help identify deficiencies in C5 function, aiding in the diagnosis of complement deficiencies.
Monitoring Disease Progression
Complement-mediated diseases, such as paroxysmal nocturnal hemoglobinuria (PNH), atypical hemolytic uremic syndrome (aHUS), can exhibit variable complement activation and dysfunction. Monitoring the functional activity of complement C5 over time can provide insights into disease progression, treatment effectiveness, and overall complement system status.
Guiding Treatment Decisions
Some complement-targeted therapies, such as mAbs for aHUS or PNH, specifically inhibit complement component C5 to mitigate complement-mediated damage.
Differential Diagnosis
The complement C5 functional test, in conjunction with other complement functional assays, can assist in differentiating between different types of complement-related disorders.
Related Products
Featured Services
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Total Complement Activity Test
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C1q-Binding Assays
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Creative Biolabs offers total complement activity test services for our clients. Total complement activity tests include CH50, AH50, and LP50.
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Creative Biolabs offers high-quality C1q-binding assays for the characterization of therapeutic mAbs.
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Learn more
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Learn more
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Protocols
Resources
As a global company, Creative Biolabs has a team of highly trained scientists to support your future projects. Creative Biolabs offers a wide range of development and research services and is wholly committed to complementing functional testing. Feel free to contact us and let us know how we can help.
References
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Horiuchi, Takahiko, and Hiroshi Tsukamoto. "Complement-targeted therapy: development of C5-and C5a-targeted inhibition." Inflammation and regeneration 36 (2016): 1-5.
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Latuszek, Adrianna, et al. "Inhibition of complement pathway activation with Pozelimab, a fully human antibody to complement component C5." PLoS One 15.5 (2020): e0231892.
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Alosaimi, Bandar, et al. "Complement anaphylatoxins and inflammatory cytokines as prognostic markers for COVID-19 severity and in-hospital mortality." Frontiers in immunology 12 (2021): 668725.
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under Open Access license CC BY 4.0, without modification
Questions & Answer
A: The complement system plays a critical role in the body's defense against infections and other diseases. Deficiencies or abnormalities in the complement system can lead to increased susceptibility to infections, autoimmune disorders, and other conditions. The complement C5 functional test helps to identify any issues with the function of the C5 protein.
A: The complement C5 functional test is typically performed on a blood sample. The sample is treated with a substance that activates or inhibits the complement system, and the activity of the C5 protein is measured using various laboratory techniques.
A: Abnormalities in complement C5 function may be associated with a range of conditions, including autoimmune disorders such as lupus and rheumatoid arthritis, as well as infections such as meningococcal disease.
A: The complement C5 functional test can be performed on both human and animal samples. However, the test optimization may be needed for different species. Depending on the volume and the quality of the sample, it is possible to conduct multiple functional tests.
A: The cost of the complement C5 functional test can vary based on a variety of factors, including the number of samples, the urgency of results, and any additional services required. We would recommend contacting our sales team for a precise quote.
A: Yes, we offer customizable testing options to meet specific experimental requirements. Simply provide us with the details and we will tailor our procedure to meet your needs.
For Research Use Only.
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