Extracellular vesicles (EVs) are nanoscale lipid vesicles secreted by cells into the extracellular matrix. In addition to containing different kinds of biomolecules such as miRNAs, mRNAs, proteins, and lipids, EVs also carry mother cell-derived cytokines. Cytokines are a class of small molecular proteins with multiple functions, mainly involved in immune cell differentiation, mediating immune response and inflammatory response, stimulating hematopoietic function and tissue repair, etc. At present, a large number of studies have only focused on the changes of cytokines in the free state in body fluids, and have not recognized the changes of cytokine in EV in body fluids. This is incomplete for understanding the cytokine profile in body fluids. Because of the stable existence of EVs in the body fluid circulation, the cytokines of EV can be regarded as a good marker. Screening the differential cytokines in EV in disease states can not only find specific disease biomarkers but also provide a new opportunity for analyzing the mechanism of disease occurrence and development. Creative Biolabs can provide isolation and purification services for EV, characterization and quantification services for EV, EV protein extraction, and cytokine profiling services of different scales to help customers analyze cytokines in EV.

Extracellular Vesicle Cytokine Detection Service

The cytokine detection service we provide is based on enzyme-linked immunosorbent assay (ELISA), which is especially suitable for the quantitative detection of a specific cytokine in a large number of EV samples. The basic principle is to combine the cytokines in EV as antigens with the corresponding enzyme-labeled antibodies. Due to the high frequency of enzyme catalysis, the final color reaction can amplify the binding reaction of cytokines and antibodies, thereby realizing the quantitative detection of cytokines in EV.

Extracellular Vesicle Cytokine Profile Detection Service

The cytokine profile detection service we provide is based on the cytokine liquid-phase suspension chip technology, which is especially suitable for the quantitative detection of multiple specific cytokines in large batches of EV samples. The basic principle is to couple highly specific antibodies to different fluorescently labeled magnetic beads, mix these antibody-labeled magnetic beads and suspend them in a 96-well microwell plate. Further, by adding a biotin-labeled high-affinity detection antibody, the target cytokines captured by the magnetic beads are simultaneously recognized. During detection, one laser beam recognizes the capture antibody labeled with magnetic beads to know which cytokines can be detected by the magnetic beads, and the other laser beam is irradiated for signal amplification detection to realize the quantification of cytokines in EV. We can provide a variety of cytokine liquid-phase suspension chips for customers to choose from according to their actual needs.

Creative Biolabs has been committed to mining the biological information in EV and can provide cytokine detection services for researchers. Whether it is the early screening of a large number of candidate cytokines and biomarkers or the large-scale verification of specific cytokines and biomarkers, you can find suitable technology platforms and services here to meet your research needs. If you are interested in EV cytokines, please contact us and make a list of cytokines you want to detect. Our professional scientific team will formulate the most suitable cytokine detection plan for you for the first time.

References

1. Kupcova Skalnikova, H.; Cizkova, J.; et al. Advances in proteomic techniques for cytokine analysis: focus on melanoma research. International Journal of Molecular Sciences. 2017. 8(12).
2. Latoch, E.; Konończuk, K.; et al. Asymptomatic survivors of childhood acute lymphoblastic leukemia demonstrate a biological profile of inflamm-aging early in life. Cancers. 2022. 14(10).

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