Exosomes play an important role in intercellular signal transduction and are a novel source of biomarkers for diagnosis and prognosis of diseases. Recently, there is evidence that cytokines are released from encapsulated exosomes, and once in contact with sensitive cells, they can cause biological effects. For exosomal cytokine detection, Creative Biolabs provide a comprehensive service, and provide a very flexible and professional choice for each specific project requirements.

Background of Exosomal Cytokines

The bidirectional communication between cells and their microenvironment is essential for maintaining the human body under physiological and pathological conditions. Cytokines are important regulators of immune function and inflammation, and are susceptible to many factors, which may lead to the high degree of heterogeneity observed in the study. In multicellular organisms, they are generally considered to be soluble factors that mediate cell-to-cell communication. Their secretion can be carried out in a typical secretion mode or through exosomes.

Fig.1 Regulation of cytokine signaling by tumor-derived extracellular vesicles.^1,3

Relationship of Exosome and Cytokines

A growing body of evidence shows that exosomes are one of the main players in cell-to-cell communication and drive inflammation, autoimmunity, and infectious disease pathology. They can be a source of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6, -1β, -8 (IL-6, IL-1β, IL-8), monocyte chemoattractant protein-1 (MCP-1) can stimulate a variety of cells to produce them. It can also induce the activation and proliferation of B cells and T cells, as well as the migration of granulocytes to inflammatory tissues, and promote the inflammatory pathway of recipient cells. These studies indicate that exosomal-related cytokines (exosomal cytokines) may be novel biomarkers.

Exosomal Cytokines Profiling Service

At present, we provide multi-parameter analysis of exosomal cytokines to provide research assistance for scientific researchers. Our platform advantages are as follows:

1. Many groups of cytokines can be detected at one time, and the required amount of samples is small. It only needs 25 μl to detect multiple indicators;
2. The cost of reagent is saved and the cost is more economical than ELISA;
3. High sensitivity, wider dynamic range than fluorescence colorimetric method;
4. Better reproducibility, less sample operation with approximately the same liquid kinetics.

Fig.2 Comparison of cytokine expression in exosomes isolated using three different methods.^2,3

Creative Biolabs is a company specialized in exosome basic research and application. For more than 10 years, we have been focusing on providing industry-leading high-quality products and professional services to our customers, striving to help them enhance their value and ability, so as to promote the vigorous development of exosome science. For more information, please feel free to contact us.

References

1. Barnes, B. J., Somerville, C. C. Modulating cytokine production via select packaging and secretion from extracellular vesicles. Frontiers in immunology. 2020, 11.
2. Jung, H. H., et al. Cytokine profiling in serum-derived exosomes isolated by different methods. Scientific reports. 2020, 10(1): 1-11.
3. under Open Access license CC BY 4.0, without modification.

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