Exosomal RNA is important for understanding the biology of the cell and can be used to identify biomarkers for disease diagnosis. Creative Biolabs provides comprehensive exosomal RNA isolation and qPCR analysis services.

Exosomal RNA Overview

• Exosomal RNA: RNA is detected in exosomes of body fluid and tissue origin and can mediate the exchange of information and metabolic activities. The characterization and quantification of exosomal RNA can be used for genomic studies, transcriptomic, epigenetic, and marker studies in cancer and other diseases.
• Exosomal RNA isolation: The extraction of RNA from exosomes allows researchers to study the nature, function and composition of these vesicles in detail. This can provide important insights into the pathophysiology of diseases, potentially paving the way for the development of new therapeutic strategies.
• Exosomal RNA qPCR analysis: qPCR analysis is an indispensable technique following the exosomal RNA isolation process. It is employed to measure the levels of specific RNAs, allowing us to gain a deep understanding of exosome-mediated gene regulation events. This method delivers high precision, sensitivity, and reproducibility results, making it the gold standard for RNA quantification.

Fig.1 The biogenesis and composition of Exosome.¹

Exosomal RNA isolation and qPCR at Creative Biolabs

Our procedure for qPCR analysis of exosomal RNA includes:

• Isolation of exosomes from a variety of sample types and then, a combination of techniques is used to isolate total exosomal RNA, including ultracentrifugation, solvent extraction, and magnetic bead separation.
• Purification of total RNA using rigorous quality control protocols.
• cDNA synthesis from total RNA.
• qPCR analysis of target genes using appropriate primers.
• Comprehensive data analysis, bioinformatics, and data interpretation.

We understand the uniqueness of each project's exosome sample depending on a variety of factors such as its source type and extraction method. We have accumulated insight into the selection of internal and external parameters in exosome RNA analysis, using suitable reference genes according to different experimental systems. This meets the high agreement between sequencing data and PCR results, while greatly improving the reproducibility of PCR results. Additionally, we are equipped with the current hot technology, known as digital PCR, which significantly improves the specificity and sensitivity of analyzing exosomal RNA while saving sample usage.

• Exosomal RNA Digital PCR Analysis Service

Fig.2 Schematic illustration of exosomal RNA qPCR analysis service.

Features

• High-Quality RNA Isolation: Our services guarantee the extraction of high-quality exosomal RNA, ensuring reliable downstream applications.
• Advanced qPCR Analysis: Our state-of-the-art qPCR analysis ensures accurate quantification and profiling of exosomal RNA, providing valuable insights into molecular mechanisms.
• Experienced Team: Our team of experts has extensive experience in exosomal RNA isolation and qPCR analysis, ensuring precise and reproducible results.
• Comprehensive Data Analysis: Along with qPCR results, we provide detailed data analysis and interpretation, facilitating meaningful conclusions for your research.

FAQs

At Creative Biolabs, our exosomal RNA isolation and qPCR analysis services aim to tailor the most appropriate approach for your research needs and provide fast and reliable results. Please contact us for more information.

Reference

1. Zhang, Huan, et al. "Exosomes as smart drug delivery vehicles for cancer immunotherapy." Frontiers in immunology 13 (2023): 1093607. Under Open Access license CC BY 4.0. The image was modified by revising the title.

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