Exosomal RNA Digital PCR Analysis Service

Overview Services Features FAQs

Droplet digital PCR (ddPCR), an emerging analytical technique in recent years, overcomes the lack of sensitivity and accuracy of traditional PCR, which is suitable for the detection of trace nucleic acids in exosomes and the analysis of copy number variant status. Creative Biolabs is committed to developing an advanced platform for exosomal components analysis and provides Exosomal RNA Digital PCR Analysis services.

Overview of ddPCR

ddPCR is a reliable and sensitive technology for quantifying and analyzing DNA or RNA samples. It is a digital PCR method that partitions a sample into thousands or millions of droplets, with each droplet serving as an individual reaction chamber.

In ddPCR, the sample is first divided into nanoliter-sized droplets using an oil emulsion system. Each droplet contains the target DNA or RNA, as well as all the necessary reagents for PCR amplification. PCR amplification then takes place within each droplet, and after the reaction is complete, the droplets are analyzed to determine whether or not they contain the target sequence.

The primary benefit of ddPCR over conventional qPCR is its capacity to quantify target nucleic acid molecules in absolute terms without the requirement for a standard curve. Because of this, ddPCR is particularly helpful for tasks requiring a high degree of sensitivity and precision, like copy number variation analysis, rare mutation detection, and gene expression profiling.

Fig. 1 ddPCR assay. (Lambrescu, et al., 2022) Fig. 1 ddPCR workflow.¹

Exosomal RNA Digital PCR Analysis Service at Creative Biolabs

Based on the absolute advantage of digital PCR in terms of sensitivity, it is especially suitable for the detection and validation of ng-level micro samples like exosomal RNA. When the amount of exosomal RNA is small, digital PCR can perform stable detection at 0.1 ng, while the detection starting amount of common RT-PCR is usually around 1 ng, which makes it difficult to complete the detection of multiple genes. Creative Biolabs has incorporated digital PCR into the sequencing validation and independent detection experiments of exosomal RNAs and established a rigorous and reliable technical route, which supports the acquisition of reliable digital PCR data.

Fig. 2 Workflow of exosome RNA digital PCR analysis service. (Creative Biolabs Original) Fig.2 Schematic diagram of exosome RNA digital PCR analysis procedure at Creative Biolabs.

Features

Comprehensive Analysis: We offer complete end-to-end solutions from isolation to analysis of exosomal RNA.
High Accuracy: Digital PCR offers higher precision and sensitivity compared to conventional methods, enabling us to detect rare transcripts with higher certainty.
Rapid Turnaround: Our optimized processes ensure the timely return of your results.

FAQs

Q: What sample types are acceptable for this service?

A: We accept a wide array of biological sample types including cells, human and animal tissue, body fluids, isolated exosomes, and purified RNA samples.

Q: How long does the full process of Exosomal RNA isolation and qPCR analysis take?

A: The standard turnaround time for this service is approximately 8-12 weeks, however, this may vary depending on the individual project requirements.

Q: What is the sensitivity of the ddPCR?

A: Using ddPCR, we can detect rare transcripts and low-abundance RNA with higher certainty. Data is highly precise and ensures greater accuracy in quantification.

Creative Biolabs offers exosomal RNA digital PCR analysis service, which is highly sensitive and has excellent reproducibility. Please contact us to discuss your project.

Reference

Lambrescu, Ioana, et al. "Application of droplet digital PCR technology in muscular dystrophies research." International Journal of Molecular Sciences 23.9 (2022): 4802. Under Open Access license CC BY 4.0. The image was modified by revising the title.

Plant Exosome Isolation