FACS based Exosome Characterization Service
Overview Services Features FAQs
Overview
Characterizing exosomes is challenging due to their small size and the lack of distinct positive populations. Leveraging cutting-edge technologies and years of expertise, Creative Biolabs provides an innovative exosome characterization platform utilizing nano-flow cytometry technology.
Exosome Characterization
Exosomes are small, membrane-derived vesicles present in bodily fluids that participate in cell-to-cell communication and regulate a wide range of biological processes. By expressing various surface markers and/or transferring biological materials directly, exosomes can influence recipient cells, playing roles in either activating or suppressing intercellular communication.
To analyze exosomes, researchers employ various methods, including immunoblotting, ELISA, and bead capture-based flow cytometry, along with advanced technologies like nano-plasmonic sensor detection. These methods typically offer bulk measurements and are unable to precisely sort distinct subsets of vesicles for detailed characterization. High-resolution imaging techniques such as cryo-EM and atomic force microscopy provide excellent resolution of exosomes but have slow throughputs and can analyze only a limited number of parameters simultaneously. Furthermore, these specialized imaging methods do not facilitate sorting and purification of exosomes for further analysis. Our nano-flow cytometry service allows for the analysis and sorting of exosomes based solely on the presence of endogenous membrane constituents.
Fig. 1 Exosomal CD63 and CD81 detection results using nano-flow cytometry.
Services
Exosome Characterization employs the nano-flow cytometry analysis technology to provide fast and accurate quantitative protein characterization and exosome sorting. Our highly experienced expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for nano-flow cytometry analysis process is listed below.
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Exosome Isolation
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Nano-flow cytometry Samples Preparation
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Sample Reading
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Data Analysis
Features
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High throughput
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Low background fluorescence
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High exosome purity
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Skillful scientific team
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Best after-sale service
With our advanced technologies and experienced scientists, Creative Biolabs excels in characterizing exosomes using nano-flow cytometry. By choosing us, you can obtain comprehensive data on your exosomes without investing in your own NTA instrument or optimizing conditions. Simply send us your sample or prepared exosomes, and we will return a detailed report with particle protein analysis data. For more information, please feel free to contact us.
FAQs
Q: Which exosome surface markers can be detected?
A: We offer three antibody markers (CD9, CD63, CD81) that can react with exosomes derived from humans, mice, and rats. Detection of CD9, CD63, CD81 in exosomes from other species or testing of other antibody markers requires customers to provide their own antibodies or to cover additional costs for antibody procurement.
Q: How much exosome sample should I provide for characterization?
A: If quantifying by particle count, you should provide at least 20μL per assay per sample, with an exosome concentration of ≥10^10 particles/mL; if quantifying by BCA, you should provide at least 5μg per assay per sample, with an exosome concentration of ≥0.25μg/μL. As exosomes from different sources can vary, it is recommended that customers provide as much sample as possible.
For Research Use Only. Cannot be used by patients.
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