Overview

Exosome Enzyme-linked Immunosorbent Assay (ELISA) is a sandwich ELISA assay designed for the quantification and characterization of exosomes in cell culture supernatant and biological fluids. The non-invasive protein biomarkers of exosomes can be used for the detection and monitoring of various pathological conditions. Creative Biolabs has successfully launched an innovative exosome characterization platform based on ELISA.

Enzyme-linked Immunosorbent Assay (ELISA)

ELISA employs the basic immunology concept of an antigen binding to its specific antibody, allowing for the detection of very small quantities of antigens such as proteins, peptides, hormones, or antibodies in a fluid sample. The assay uses enzyme-labeled antigens and antibodies to detect biological molecules, with alkaline phosphatase and glucose oxidase being the most commonly used enzymes.

Services

ELISA captures exosomes using exosome-specific protein antibodies from various biological samples, including cell culture supernatant and biological fluids. Following capture, the quantification and characterization of exosome proteins are performed using appropriate detection antibodies to identify exosome-associated antigens, which can be generic or cell-specific. Exosomes perform different biological functions due to their varied protein compositions. For instance, tumor cell-derived exosomes can mediate the proliferation and immune escape of angiogenic tumor cells, while dendritic cell-derived exosomes can elicit effective anti-tumor immune responses. Therefore, the non-invasive protein biomarkers of exosomes can be used for the detection and monitoring of multiple pathological conditions.

Features

• No initial exosome purification required
• Feasible for analyzing multiple markers
• High throughput
• Skillful scientific team
• Excellent after-sale service

With our well-established technologies and experienced scientists, Creative Biolabs offers customized ELISA services for customers worldwide. We have a vast array of antibody resources to meet the needs of exosomes from different sources. Send us fresh cell culture supernatant and biological fluids for exosome detection, and we will provide a complete data report including experimental procedures, methods, reagents, instruments, standard curves, and data analysis. We offer highly flexible options for each specific case and are happy to make our services accessible to all types of research and industrial customers. Please do not hesitate to contact us for more information.

FAQs

• FACS
• Western Blotting