Creative Biolabs offers a high-throughput and highly accurate MRM/PRM targeted exosomal proteomic quantitative service that can validate these exosome-based markers in large biological sample sizes based on differential proteomic data.

Introduction of MRM/PRM Targeted Exosomal Proteomic Quantitative

• Targeted quantitative proteomics uses mass spectrometers for ion screening and targeted detection of fragmented ions from selected exosomal peptides.
• There are now two primary technical paths that are being followed: PRM (Parallel Reaction Monitoring) and MRM (Multiple Reaction Monitoring).
• Using triple-quadrupole mass spectrometers, MRM is intended for the focused examination of certain proteins, peptides, or tiny compounds in complicated materials.
• PRM uses high-resolution/precision mass spectrometers and is a simplified version of MRM.

Fig. 1 MRM/SRM and PRM targeted proteomics.¹

MRM/PRM Targeted Exosomal Proteomic Quantitative Service at Creative Biolabs

We offer one-stop MRM/PRM exosome quantitative protein analysis services, including MS analysis, raw data analysis, bioinformatics analysis, specific peptide selection, isotope-labeled peptides, and MRM/PRM method establishment and optimization, based on the target protein information you provide for the desired study. There are several facets of bioinformatics analysis available, including

• Spectrogram analysis, and protein data quality assessment.
• PCA analysis of multi-component samples.
• Functional annotation of identified proteins: GO functional annotation, KEGG functional annotation, and COG functional annotation.
• Venn diagram and volcano plot statistical analysis of proteins with differential expression.
• K-means clustering and hierarchical clustering for the study of proteins with differential expression.

Fig. 2 MRM/PRM workflow.

Features and Applications of MRM/PRM

1. MRM assay

• Utilizes a triple quadrupole mass spectrometer for targeted large-molecule protein detection.
• Benefits:

Reduces interference from other ions.

Improves signal-to-noise ratio, sensitivity, and reproducibility.

Suitable for high-throughput monitoring of marker proteins.

• Can achieve absolute quantification by adding isotopically labeled peptides as internal references.
• Optimal for measuring particular sets of target exosomal proteins in large-scale specimens.

2. PRM assay

• Relies on high-resolution mass spectrometers for targeted large-molecule protein detection.
• Features:

Single mass spectrometer for proteome screening and validation.

Easy operation and low detection cost.

Can target a large number of ions for monitoring.

• Challenges:

Instrumentation can be less stable due to nanoliter liquid phase system.

Requires fine adjustment of MS acquisition parameters for accuracy and precision.

• Typically used shortly after non-targeted proteomics and before mass spectrometer maintenance.
• Appropriate for exosomal protein samples in tiny batches that necessitate protein-targeted quantitative assays.

FAQs

Using high-resolution, high-precision mass spectrometry, Creative Biolabs provides MRM/PRM focused exosomal proteomic quantitative services that facilitate the selective identification and accurate quantification of exosomal proteins. Kindly get in touch with us for a quote.

Reference

1. Guerrero, Laura, Alberto Paradela, and Fernando J. Corrales. "Targeted Proteomics for Monitoring One-Carbon Metabolism in Liver Diseases." Metabolites 12.9 (2022): 779. Under Open Access license CC BY 4.0. The image was modified by revising the title.

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