Respiratory Disease Model Construction Service for Exosome Functional Research

There are many diseases of the respiratory system, the most common being pulmonary fibrosis (PF), chronic pulmonary obstruction (COPD), bronchopulmonary dysplasia (BPD), asthma, and other lung diseases. These diseases will affect breathing and cause symptoms such as coughing, expectoration, wheezing, and difficulty breathing. If not treated in time, these diseases will lead to respiratory failure and even death. In recent years, a large number of studies have found that natural or engineered exosomes have good therapeutic effects on these respiratory diseases. Creative Biolabs has been focusing on the development and progress of therapeutic exosomes that can treat respiratory diseases. With years of accumulated experience, we have established a complete animal model library that can better simulate clinical conditions, which can help customers study the mechanism of exosomes in the occurrence and development of respiratory diseases or detect the effectiveness of therapeutic exosomes in the treatment of respiratory diseases.

Smoking room. (Ridzuan, et al., 2021) Fig.1 The smoking chamber.^1,2

Creative Biolabs Respiratory Disease Animal Model Library for Exosome Functional Research

We can provide including but not limited to the following respiratory disease animal models for exosome functional research.

Respiratory Disease Animal Models	Inducer	Induction Mechanism	Applicable Animals	Model Features
PF animal models	Bleomycin	Bleomycin can induce DNA breakage, free radical generation, and oxidative stress, which can cause apoptosis or necrosis of lung cells, thereby leading to lung inflammation and fibrosis.	Mouse, rat	Bleomycin can induce the proliferation of fibroblasts and the diffusion of collagen fibers in the lung interstitium, and significantly destroy the alveolar structure.
COPD animal models	Cigarette smoke (CS)	Smoke from a burning cigarette contains carbon monoxide, nicotine, and tar. These harmful substances enter the body to cause hypoxia and vascular damage and directly inhibit intracellular respiration and damage lung tissue. In addition, smoking suppresses immune function and damages the airway-ciliated mucus clearance system, making it prone to infection by pathogenic microorganisms.	Mouse, rat, guinea pig	CS induces chronic airway inflammation, mucus hypersecretion, airway remodeling, and emphysema.
COPD animal models	CS + lipopolysaccharide (LPS)	The lung inflammation caused by LPS can further suppress the immune function of the body and accelerate the progression of COPD.	Mouse, rat	Compared with simple CS induction, CS+LPS induction can shorten the modeling time and significantly weaken the resistance of experimental animals to CS.
BPD animal models	LPS	In the body, LPS can synthesize and release various cytokines and inflammatory mediators by activating monocyte-macrophages, endothelial cells, epithelial cells, etc., and then cause lung inflammation.	Mouse, rat	These inducers can lead to alveolar structural disorder, alveolar rupture, and infiltration of inflammatory cells around the alveolar space and pulmonary septum.
BPD animal models	High of concentration oxygen	High concentration of oxygen leads to the generation of a large number of unsaturated lipids in the lungs, which interferes with intracellular enzyme metabolism, thereby destroying the structure and function of lung cells.	Mouse, rat
Asthma animal models	Ovalbumin (OVA)	OVA can induce Th2 sensitization and produce various pro-inflammatory cytokines. These pro-inflammatory factors can induce the production of allergen-specific immunoglobulin E and chemokines, and the secretion of eosinophils.	Mouse, rat, guinea pig	These models can reproduce the classic symptoms of asthma, such as airway hyperresponsiveness, chronic airway inflammation, and increased mucus.

Creative Biolabs is a CRO company specializing in exosome research and development. Based on operating specifications that comply with international standards, we can provide customers with the most comprehensive respiratory disease model construction services to help the development of respiratory disease treatments. In addition, we can also provide follow-up nucleic acid and protein testing, histological testing, and other services. If you want to further study the functional mechanism of exosomes in respiratory diseases or the efficacy of therapeutic exosomes in vivo to accelerate the transformation of results, please contact us to put forward your ideas. We will formulate the most suitable experimental plan for you.

References

Ridzuan, N.; et al. Human umbilical cord mesenchymal stem cell-derived extracellular vesicles ameliorate airway inflammation in a rat model of chronic obstructive pulmonary disease (COPD). Stem Cell Research & Therapy. 2021, 12(1):54.
under Open Access license CC BY 4.0, without modification.

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