Tumor Model Construction Service for Exosome Functional Research

Overview Disease Model FAQs

Overview

As small extracellular vesicles containing a variety of biologically active substances, exosomes are almost involved in the entire process of tumor development and are closely related to tumor progression. However, exogenous exosomes can be used as carriers of tumor therapeutic drugs to target anti-tumor components such as loaded nucleic acids, proteins, lipids, and small molecular compounds to tumor cells to achieve tumor cell killing. A large number of studies have shown that natural exosomes or engineered exosomes can treat tumors by inhibiting tumor cell proliferation and metastasis, regulating tumor microenvironment, inhibiting tumor angiogenesis, regulating tumor drug resistance, and activating immune cells. Therefore, exosome therapy is expected to become a new and efficient way of tumor treatment. Experimental animal models have long been used as an important tool for studying human diseases and developing new drugs. Animal models currently available for tumor research mainly include induced tumor animal models, transplanted tumor animal models, and genetically engineered mouse models. These models can be used for screening and efficacy studies of new exosome drugs. Creative Biolabs can provide various tumor animal model construction services and in vivo efficacy evaluation services to help customers study the efficacy and safety of therapeutic exosomes.

HELA-Exos activate DCs in a mouse xenograft model. (Huang, et al., 2022) Fig.1 Mechanism by which HELA-Exos activate DCs in situ in a mouse xenograft model of TNBC.^1,2

Creative Biolabs Tumor Disease Model Library for Exosome Functional Research

We can provide including but not limited to the following tumor disease animal models for exosome functional research.

Tumor Animal Models	Induction Mechanism	Applicable Animals	Model Features
Induced tumor animal models	Under experimental conditions, tumors are induced in animals by using physical, chemical, and biological carcinogens. The most common chemical carcinogens mainly include benzopyrene, methylcholanthracene, benzidine, nitrosamines, and aflatoxins.	Mouse, Rat	The inducing factors and conditions of this model can be controlled artificially, and the inducing rate is much higher than the natural incidence rate.
Cell-line-derived tumor xenograft animal models	Tumor cell lines are transplanted into allogeneic or xenogeneic animals to form tumors. The transplantation site is generally subcutaneous, orthotopic, or systemic injection.	Mouse	This model is often used for anticancer drug screening and efficacy evaluation.
Human tumor tissue xenograft animal models	Fresh tumor tissue from patients is directly inoculated Immunodeficient mic. The site of inoculation is usually subcutaneous, in situ, or systemic injection.	Mouse	This model retains the stromal heterogeneity and histological characteristics of the patient's tumor, can provide an in vivo simulation environment for tumor research, and can more objectively and comprehensively reflect the tumor's response to drug effects.
animal models	Specific genes are knocked out or inserted by using techniques such as transgenic, gene targeting, and conditional gene targeting, resulting in the spontaneous formation of tumors in vivo.	Mouse	This model has great advantages for studying the mechanism of tumorigenesis and tumor immune evasion.

Creative Biolabs can provide tumor and cancer-targeted exosome modification services, exosome loading services, and corresponding tumor animal model construction services according to your needs. At the same time, we can also provide you with other exosome research services, including exosome extraction, exosome identification, exosome engineering, exosome labeling, and in vivo and in vitro verification of exosomes, which can meet your various needs for exosome drug development. If you want to develop exosome drugs for tumor treatment, please contact us without hesitation. Our professional team will respond to your inquiry as soon as possible and formulate the most cost-effective overall experimental plan for you.

References

Huang, L.; et al. Engineered exosomes as an in situ DC-primed vaccine to boost antitumor immunity in breast cancer. Molecular Cancer. 2022, 21(1):45.
under Open Access license CC BY 4.0, without modification.

FAQs

What is the significance of exosomes in tumor development, and how can they be utilized for tumor model construction?

Exosomes play pivotal roles in tumor progression, metastasis, and intercellular communication within the tumor microenvironment. Our service leverages exosomes to construct tumor models that recapitulate key features of tumor biology, facilitating the study of tumor progression and therapeutic interventions.

How are exosomes sourced for tumor model construction, and what advantages do they offer in this context?

Exosomes can be isolated from tumor cells, patient-derived samples, or tumor-conditioned media, serving as carriers of tumor-specific biomolecules and signaling molecules. Utilizing exosomes for tumor model construction offers advantages such as physiological relevance, representation of tumor heterogeneity, and the ability to recapitulate tumor-stroma interactions accurately.

Which types of tumors can be modeled using exosomes, and how accurately do these models mimic human tumor biology?

Our service enables the modeling of various tumor types, including breast cancer, lung cancer, melanoma, and glioblastoma, among others. These models closely mimic human tumor biology by incorporating tumor-derived exosomes containing molecular cargo reflective of tumor phenotype, facilitating the study of tumor progression and therapeutic responses.

What methodologies are involved in constructing tumor models using exosomes, and what experimental approaches are employed?

Tumor models are constructed by administering tumor-derived exosomes to relevant in vitro or in vivo model systems, such as cell cultures or animal models. Experimental approaches include exosome uptake assays, functional assays, histological analysis, and molecular profiling techniques to assess tumor-relevant phenotypic changes and therapeutic responses accurately.

Can these exosome-based tumor models be customized to address specific research questions or therapeutic targets?

Yes, our service offers customizable tumor models tailored to address specific research questions, therapeutic targets, or experimental requirements. Researchers can choose tumor-specific exosome sources, model systems, tumor subtypes, endpoints, and outcome measures, allowing for flexible study designs and the exploration of diverse research avenues.

Plant Exosome Isolation