Breast Cancer-targeting Adenovirus Vector Construction Service
Creative Biolabs is a leading company in the field of molecular cell biology tools, and we have always helped researchers around the world with high-quality products and services. In response to the current research direction of gene therapy, we have established a comprehensive adenovirus vector construction service platform to meet all your scientific and commercial needs by designing or improving gene delivery vectors with different functions and characteristics. No matter what difficulties you have encountered in the relevant research work, you can contact us to help promote your project.
Breast Cancer-targeting Gene Therapy
Breast cancer is a typical cancer caused by a series of genetic mutations. It has always been the focus of gene therapy. Scientists hope that a nucleic acid-based drug can be used to correct or destroy cancer cells with abnormal genes. The biggest difficulty of this treatment is how to deliver the nucleic acid to the right place. Since DNA is easy to form circulating nucleases in cells, its own ability to transmit is very limited, and it is necessary to achieve the purpose of gene therapy through the help of vectors.
In theory, there are many targets for breast cancer that can be used as gene therapy, but most of the current treatment research is still in the clinical trial stage. The strategies used in these treatments are divided into four aspects: enhancing their own immune response, transferring some suicide genes to promote cancer cell self-destruction, using the drug resistance gene to protect bone marrow cells, inhibiting the cancer cells' proliferation and metastasis by repair or activation of tumor suppressor genes. All of these gene therapies need to overcome many biological barriers both extracellularly and intracellularly. Therefore, in order to achieve successful nucleic acid transfer, a safe and efficient gene transfer vector must be designed.
Adenovirus Vector for Gene Therapy
The virus can use its own genetic characteristics to efficiently transduce cells, providing an ideal delivery system for gene therapy. Recombinant viruses are generally non-replicable and require a packaged cell line to be produced and genetically engineered to carry specific therapeutic genes. The characteristics of the virus should match the disease being treated. As one of the most widely used human cell gene transfer vectors, adenovirus has more than 50 serotypes in humans and is widely distributed. Adenovirus has rich genetic diversity, extensive tissue tropism, and its genomic characteristics are obvious, so genetic manipulation is very convenient. Because of these advantages, adenovirus has demonstrated its potential as a safe and reliable carrier since its use in gene therapy research.
Replication-defective adenoviral vectors are constructed to carry "suicide" genes that can kill the cell in which they reside if the genes are activated. These vectors are constructed such that the suicide genes are not active in any vital organs and are expressed in a tissue-specific manner in breast cancer. The tissue specificity is accomplished by cloning specific gene promoters into the vectors. These promoters are cloned from genes which are expressed almost exclusively in breast tissue and breast cancer tissue. Therefore, the expression of genes driven by these promoters is essentially tissue specific. Tissue specificity is demonstrated in tissue culture and in mice. Adenovirus vectors are also deleted in both E1 and E3 regions to maximize the foreign DNA cloning capacity, facilitating gene therapy of breast cancer by targeting breast cancer cells and killing them.
Creative Biolabs has a rich product library and R&D service platforms. Various biological research tools for cancer treatment have always been the focus of our work. To help your research go smoothly and efficiently, we have launched an adenoviral vector construction service specifically for breast cancer, which can achieve efficient and long-lasting expression of the target gene. If you have any question, please contact us by email or send us an inquiry to find a complete solution.
