Neurons-targeting Adenovirus Vector Construction Service
Through our comprehensive early discovery, nonclinical and commercialization services, Creative Biolabs is committed to developing the most promising viral vectors for our clients. With in-depth expertise in vector design, vector construction, as well as in-vivo and in-vitro studies, we can provide a series of adenovirus vector services basing on a full range of state-of-art technologies. Nowadays, we provide high-quality customized assays, with different formats, endpoints, parameters, to satisfy any specific requirement at a most competitive price.
Introduction of Adenovirus Vector
Adenoviruses (Ads), a member of the Adenoviridae family, are the main cause of many respiratory illnesses, including pneumonia, respiratory tract infection as well as conjunctivitis. Ads have a linear DNA genome with a length of 36000 based pairs, and the genome is responsible for encoding about 35 proteins in the process of DNA replication. In the past few years, a broad range of adenovirus serotypes have been identified, for instance, more than 57 serotypes of human Ads have been defined by using cell cultures targeting different kinds of anti-sera. Meanwhile, pilot studies have also shown that adenovirus vector is an attractive delivery system for gene transfer in non-dividing or dividing cells. A number of adenovirus vectors have been developed for treating various diseases, especially for cancers. The results in clinical trials suggest that adenovirus vector is a safe and effective gene transfer system with high viral titers. Currently, most Ad vectors have been constructed by modifying Ad2 or Ad5, for instance, the replication-defective Ad5 vector and the replication-competent Ad5 vector have been widely used for neurons-associated disease therapy.
Figure 1. Schematic diagram of shuttle vectors used for the construction of recombinant adenoviruses. (Southgate, 2008)
Service
Adenovirus vectors play an important role in mediating a wide variety of disease treatment and provide a novel strategy for gene therapy. More recent studies indicate that adenovirus vectors have been utilized to transfer genes into the central nervous system. These vectors are capable of enhancing the gene expression and studying the physiological functions of certain genes in the brain. As a result, the use of adenovirus vectors targeting neurons should be essential for establishing specific methods in many gene therapy applications. Now Creative Biolabs has developed a series of services to design and construct adenovirus vectors targeting neurons for our clients. E1A and E1B deleted adenovirus vectors are commonly used for expressing foreign transgenes in our labs. These vectors can be cultured in many kinds of cells lines, including HEK293. In addition, we have successfully transferred genes into the neurons and glial cells by using Ads. The transgene expression has been evaluated by a wide collection of assays, such as fluorescence-activated cell sorting (FACS) assay, enzyme-linked immunosorbent assay (ELISA) and real-time PCR assay. Up to now, we have established protocols to clone, infect, and test neurons-targeting adenoviral vectors to ensure their safety and efficacy in gene therapy.
Figure 2. Neurons-targeting adenovirus vector construction service.
As a global contract research organization, Creative Biolabs works on a wide range of viral vector discovery and development services for various disease types. We utilize unrivaled, proprietary vector design, study data, product candidate, advanced project life-cycle management, as well as real-time data to ensure the ideal outcomes. If you are interested in our services, please feel free to contact us for closer communication to learn how we can be involved in your project. Separate services or integrated end-to-end solutions are all welcomed.
Reference
- Southgate, T.; et al. (2008). Gene transfer into neural cells in vitro using adenoviral vectors. Curr Protoc Neurosci. 4: 23.
