GTOnco™ T Cell Activation & Proliferation Assay
The stimulation of antigen leads to a series of immune responses. During the immune response, the activated T lymphocytes undergo rapid expansion to target and attack the tumor. The interaction between activated immune cells themselves with antigen-presenting cells is an important part to regulate the immune responses. Therefore, it is vital to understand the mechanisms of immune cell activation, proliferation and regulation in gene therapy-based I-O drugs development. Based on the outstanding expertise and rich experience, Creative Biolabs has the ability to conduct top-quality T cell activation and proliferation assays to validate the anti-tumor activity of your I-O drugs. We provide the best-fit methods and commercial kits for our clients' selection.
T cell activation is initiated by the engagement of TCR and CD3 complex, and the subsequent engagement of co-stimulatory molecules, such as CD28 receptor. After co-stimulation of these two signals, a series of intracellular activations happen, including the cytokine release and T cell proliferation in order to fight infection or disease. Scientists at GTOnco™ have developed a systematic approach to T cell activation and proliferation assays for I-O products discovery. The nuclear factor of activated T cells (NFAT), IL-2 or other kinds of factors will be detected by bioluminescent methods or ELISA kits. Meanwhile, the T cell activation and proliferation process can also be evaluated via cell counting kit-8 (CCK-8), MTT, CFSE as well as flow cytometric analysis, etc. We guarantee to provide the best-class service for our clients during the gene therapy-based I-O drugs development.
Features of Our Services
- Extensive experience and skillful technicians
- Advanced and custom T cell activation and proliferation assays are available
- Real-timely visualize and quantify the interaction and proliferation of immune cells
- Superior quality and timely feedback
Creative Biolabs is a leader in the field of I-O products discovery with great reputation and experience. We are dedicated to providing professional and advanced in vitro assay platform for our customers around the world. For more detail information, please feel free to contact us.
Reference
- Andrew Lichtman. (2017). T Cell Mediated Immunity: Selected Features Relevant to Cancer Immunotherapy.
