Tetracycline-inducible Adenovirus Vector Construction Service

Tetracycline inducible adenovirus vectors play an important role in gene function and gene therapy research for their various advantages over other vector systems. As a leading provider of adenovirus vector, Creative Biolabs offers a full range of tetracycline-inducible adenoviral vector construction services based on our powerful adenovirus vectors technology platform and experienced experts.

Introduction of Tetracycline-inducible Expression System

In basic research, control of transgene expression in mammalian cells is critical for gene therapy and gene function research. Several regulated systems have been used to control transgene expression, including tetracycline, ecdysone, antiprogestin and dimerizer-based systems. Among these systems, tetracycline-inducible expression system has several advantages over other regulated gene expression systems, such as tight on/off regulation, high inducibility, fast reaction times and no pleiotropic effect due to the use of the tetracycline operon derived from bacteria.

The tetracycline-inducible expression system includes two types: Tet-off and Tet-on. In the Tet-off system, the tetracycline-responsive transcriptional activator (tTA) induces the transcription of a gene containing the Tet-responsive element. The transcription is turned off in the presence of tetracycline. In the Tet-on system, the reverse tetracycline-responsive transcriptional activator (rtTA) binds to the Tet-responsive element and turns on the transcription in the presence of tetracycline. Thus, the Tet-off and Tet-on systems provide a negative and positive control of transgene expression, respectively. In general, the Tet-on system is superior to Tet-off for gene therapeutic applications because its responsiveness is faster and it does not require continuous pharmacological treatment after gene expression termination.

Tetracycline inducible regulatory systems. (A) Tet-OFF regulatable switch. (B) Tet-ON regulatable switch.Figure 1. Tetracycline inducible regulatory systems. (A) Tet-off regulatable switch. (B) Tet-on regulatable switch. (Goverdhana, 2005)

Construction of Tetracycline-inducible Adenoviral Vectors

Adenoviral vectors are preferred vectors for gene therapy and gene function studies. Because they are relatively easy to construct and can be produced at high titers, and have the ability to tightly regulate transgene expression. The tetracycline-inducible system for gene expression (especially the Tet-on system) is one of the most valuable tools for controlling gene expression. The combination of adenoviral vectors and tetracycline-inducible expression system is clearly an advantage for gene therapy and gene function studies. Adenoviral vectors can transfer genes to a wide range of mammalian cells in vitro and in vivo with nearly 100% efficiency. By coinfecting the vector expressing tTA or rtTA with the vector expressing the gene of interest under the control of a Tet-responsive promoter, the tetracycline-inducible system can be easily controlled not only in a plurality of cultured cells, but also in vivo in animals. Adenoviral vectors containing the tetracycline-inducible expression system provide tightly-controlled gene expression and have proven to be an attractive strategy for gene therapy and basic analysis of gene function.

Service

Creative Biolabs combines the tightest and sensitive gene expression control systems with the most advanced adenoviral vector systems to construct tetracycline-inducible adenoviral vectors. Using this combination, the tightly-controlled inducible expression is as easy as constitutive expression, and cloning into an adenoviral vector is as simple as cloning into any plasmid. In order to better meet the needs of each customer, we provide custom services for each step of the tetracycline-inducible adenovirus construction process.

Features

  • Very tight control of gene expression
  • Simple-to-use tetracycline-inducible system
  • The most advanced adenoviral vector construction technology

In addition to the above services, other regulated adenoviral vector construction services are also available at Creative Biolabs, including:

If you would like to know more about the construction of adenoviral vectors, please feel free to contact us.

Reference

  1. Goverdhana, S.; et al. (2005). Regulatable gene expression systems for gene therapy applications: progress and future challenges. Molecular Therapy. 12(2):189-211.
For research use only. Not intended for any clinical use.