Dodecanyl-Liposome (CAT#: CLBD023LY)

Description
Dodecanyl-Liposome is Liposome with the carboxyl group, which can be activated by EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide) and react with sulfo-NHS (N-hydroxysulfosuccinimide) to produce a significantly more stable and more soluble active intermediate (NHS ester). The intermediate can covalently conjugate to the amine group on the proteins, peptides or antibodies through displacement of sulfo-NHS group by amine.
Lipid Composition
L-alpha-Phosphatidylcholine, Cholesterol, 18:1 Dodecanyl PE
Buffer
PBS
pH
6
Storage
Store in dark at +4°C and do not freeze
Size
100 nm
Quantity
5mL (available in lyophilized powder)
Molecular Structure
Download
DataSheet MSDS
FAQs Customer Reviews Related Sections
  1. What is the role of EDC in the activation process?

    EDC is a zero-length crosslinking agent that reacts with carboxyl groups to form an amine-reactive intermediate, O-acylisourea.

  2. What is the optimal pH range for the EDC/sulfo-NHS activation reaction?

    The activation reaction is most efficient at a pH range of 4.5-7.2.

  3. Why is sulfo-NHS added to the activation process?

    Sulfo-NHS is added to stabilize the intermediate formed by EDC, preventing hydrolysis and enhancing solubility.

  4. Why should Tris buffer not be used in the process?

    Tris buffer contains amine and should not be used as it can interfere with the amine-reactive intermediate.

  5. What are the storage conditions for EDC and sulfo-NHS before use?

    They should be prepared immediately before use and kept at room temperature.

  • Enhanced Antitumor Activity
    Using Dodecanyl-Liposome in our cancer research has tremendously improved the antitumor activity of our treatments.
  • High Efficiency
    The highly efficient coupling of antibodies to Dodecanyl-Liposome has significantly streamlined our workflow.
  • Clear Instructions
    The detailed instructions provided by Creative Biolabs made the activation and conjugation process straightforward.
  • Preserved Activity
    The biological activity of our proteins was fully preserved using Dodecanyl-Liposome for conjugation.
  • Optimal pH Formulation
    The PBS buffer with pH adjusted to 6 is ideal for our coupling reactions.

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For Research Use Only. Not For Clinical Use

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