NAA Services for Anti-Acetylcholine Receptor (AChR)

Having worked in the field of natural autoantibody (NAA) research for many years, Creative Biolabs has successfully developed a series of innovative and diversified NAA platforms to provide fast and convenient services for our worldwide customers. Now, we are capable to provide a comprehensive set of services against anti-acetylcholine receptor (AChR) biomarker for diseases diagnosis and therapeutic monitoring. Our professional and experienced scientists will take part in every step of your program to accelerate your project development and do their best to meet your requirements.

Background of Anti-acetylcholine Receptor (AChR) Antibody

Mammalian nicotinic acetylcholine receptors (nAChRs) are composed of five subunits arranged around a water-filled pore. Three types of autoantibodies against AChR of skeletal muscle are detectable in patients with myasthenia gravis (MG) including binding, blocking, and modulating anti-AChR autoantibodies. Modulating autoantibodies correlate best with the severity of the disease, but are also technically most difficult to measure because the assay generally requires fresh human muscle cells.

Neuronal nAChR structure. Fig.1 Neuronal nAChR structure. (Hendrickson, 2013)

The Role of Anti-AChR Antibody in Myasthenia Gravis (MG)

MG is an autoimmune disease limited to specific organs in which autoantibodies attack nAChRs in neuromuscular junctions. A diagnosis of MG is based on pathognomonic history, clinical characteristics, and the results of necessary examinations. In cases of mild symptoms or questionable examination results, diagnosis relies more on pathognomonic history. Thus, it is difficult to diagnose patients whose medical histories have been poorly recorded. Normally, the repetitive nerve stimulation test, the edrophonium test, anti-AChR autoantibody titers are used for MG diagnosis. It is worth mentioning that an abnormal increase in anti-AChR autoantibodies titer is observed in more than 90% of cases of MG. Now, anti-AChR autoantibodies have become an ideal biomarker of MG with superior diagnostic accuracy, and it is widely-accepted that antigen-specific apheresis of anti-AChR autoantibodies seems to be a safe and effective treatment for MG that can be scaled up for clinical testing.

Healthy neuromuscular transmission and the MG neuromuscular junction. Fig.2 Healthy neuromuscular transmission and the MG neuromuscular junction. (Phillips, 2016)

Anti-AChR Autoantibody Detection

A solid-phase enzyme immunoassay for the measurement of anti-AChR autoantibodies in the sera of patients with MG is reported and the correlation between antibody titers measured by enzyme immunoassay and the immunoprecipitation assay was significant. Based on years of extensive experience in NAA research, We are equipped with different approaches for NAA analysis, including but not limited to protein array, peptide array, ELISA, LIPS, WB and dot blot.

What We Can Do about NAA?

Equipped with well-established platforms and experienced scientists, we can provide comprehensive NAA services, from NAA detection, NAA profiling, to NAA epitope mapping. A wide spectrum of NAA products is available for your choice.

Features of Our Services

Creative Biolabs is well-positioned to deliver focused NAA services and products to the research market. We also have extensive expertise and experience to meet the customized requirements of your research and project. Our proven and optimized platforms can help you quickly get satisfactory results without repeated trials, please do not hesitate to contact us for more details.

References:

  1. Hendrickson, L.M.; et al. Neuronal nicotinic acetylcholine receptors: common molecular substrates of nicotine and alcohol dependence. Frontiers in psychiatry. 2013, 4: 29.
  2. Phillips, W.D.; Vincent, A. Pathogenesis of myasthenia gravis: update on disease types, models, and mechanisms. F1000Research. 2016, 5: 1513.
For Research Use Only | Not For Clinical Use

Related Services:

Products:

Resource:

Applications:

Online Inquiry
Inquiry