As a revolutionary technology in the field of gene editing, CRISPR has been widely used in basic and medical research. Creative Biolabs has previously employed CRISPR/Cas9 technology to generate innovative and effective cell therapy services for our customers. Aided by our CRISPR/Cas9 platform, you can revolutionize your particular project by enabling any site-specific genome editing without introducing foreign DNA.
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) system is historically recognized as an adaptive immune system that protects bacteria and archaea from viral (phage) and plasmid infection. But in recent years, CRISPR/Cas9 is more famous as one outstanding scientific breakthrough in genome editing. It can mediate gene modification at particular locations to help scientists edit the genomes of a variety of organisms rapidly and efficiently, which also provides the potential for cell therapy applications. There are several important components involved in the CRISPR/Cas9 system, among which Cas9 and sgRNA (synthetic single-guide RNA) are essential in genome editing: sgRNA is responsible for the site targeting, and Cas9 contributes to the DNA cleavage at the target site. Usually, the RNA-guided Cas9 associates with the dual RNA guides: crRNA and tracrRNA (separately represent CRISPR RNA, trans-activating RNA) or sgRNA, subsequently recognizes and cleaves the specific DNA sequence complementary with the protospacer. CRISPR/Cas9 has been explored in research on various diseases, including single-gene disorders such as cystic fibrosis, hemophilia, and sickle cell disease. Furthermore, CRISPR/Cas9 has great promise in the cell therapy of complex diseases, including cancer, heart disease, mental illness, HIV infection, etc.
Fig 1. Schematic of (epi) genome editing by CRISPR-Cas9.
With the development of CRISPR/Cas9 system, this innovative technology has attracted massive attention and has been applied to a variety of model organisms. Creative Biolabs offers a wide range of products and services about CRISPR/Cas9 for our clients.
1) gRNA Design and Vector Construction Service
Careful design and construction of guide RNAs (gRNAs) and homologous recombination (HR) donors is an important first step in CRISPR/Cas9 technology. Creative Biolabs can help you handle this first step to save yourself time and trouble. We offer target sequence cloning service into appropriate vectors, including all in one CRISPR/Cas, T7 vector, and lentiviral transfer vectors. There are two options for you:
We usually offer two gRNAs at least, and targets can destroy important domains of the protein and all the alternatively spliced transcripts. The knockout efficiency of our vector is very high.
2) CRISPR/Cas9 Cell Line Engineering
Creative Biolabs provides a variety of reliable CRISPR/Cas9 cell lines engineering services to produce a genetically modified cell using any mammalian cell line and targeting any gene.
3) CRISPR/Cas9 Genome Knockout Kits
Creative Biolabs offers genome-wide CRISPR gene knockout /knockin kits containing 2 gRNA vectors and donor DNA to help you modify the specific gene by yourself. The kits are ready-to-use and highly efficient with non-homology mediated gene knockout.
4) Transgenic Mice Services
Creative Biolabs can also offer transgenic mice with CRISPR/Cas9-mediated genome modifications.
Fig.2 Generation of knockout or knock-in mice using CRISPR/Cas9 in embryonic stem (ES) cell. (Burgio, 2018)
CRISPR/Cas9 is a simple and efficient genome editing tool. Aided by our well-established platforms and experienced scientists, Creative Biolabs has successfully completed dozens of genome engineering projects using CRISPR/Cas9. Because each project is different, if you don't see the CRISPR/Cas9 service you need above, please contact us, we can customize our offering to meet your specific project needs.
Reference
For Research Use Only. Not For Clinical Use.