Overview Service Features FAQs Scientific Resources Related Services
Overview
Nowadays, human induced pluripotent stem cells (iPSC) offer great resources for application in pharmaceutical development and regenerative medicine. With years of exploration in iPSC development, Creative Biolabs is dedicated to providing several iPSC culture services, including Maintenance of iPSC, 3D culture of iPSC, as well as scale up culture of iPSC.
As we all know, the growth of any type of mammalian cell in vitro needs extracellular matrices, growth media, and environmental factors, as well as iPSC. However, the iPSC culture has several specific factors need took into consideration, which may influence the quality, robustness, and utility of a variety of iPSC culture methods.
Growth Medium Development for iPSC Culture
Growth medium plays the most important part in hPSC culture, and goes through a dynamic evolution since it was first used for hESC culture. The final goal for therapeutic application is to design a serum-free, xeno-free, and chemically defined medium, available for supporting the growth of almost all types of iPSC lines. Recently, researchers have developed chemically defined E8 medium, a derivative of TeSR1 containing 8 components. This novel medium is short of both serum albumin and β-mercaptoethanol. This E8 medium, combined with EDTA passaging, is a promising culture for a great number of iPSC and hESC lines, it also may promote episomal vector-based reprogramming efficiencies and experimental consistency.
Fig 1. Generation of different organoids using iPSCs in 3D environments. (Li, Y., 2017)
Extracellular Components
Extracellular components comprise a variety of organic matrices from animal cells, individual matrix proteins, hydrogel, synthetic surfaces, as well as some commercially well-defined and xenogeneic-free components.
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Matrigel: It is an extensively used extracellular component for feeder-free culture of hPSCs. Mostly, it can find in types I and IV collagens, heparan sulfate proteoglycan, laminin, undefined growth factors, entactin, matrix, as well as metalloproteinases.
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Extracellular matrix (ECM) proteins: Most ECM proteins are regulated by development, and some of them are able to be used to support hPSC self-renewal or lineage commitment. For example, recombinant vitronectin is a defined substrate which enables to sustains hESC self-renewal via adhesion with αVβ5 integrin.
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Synthetic surfaces: It is considered that synthetic surfaces can mimic major signal transduction pathways which are needed for hPSC growth. Besides, modifications of cell growth surfaces with simplified methods can enhance the development of chemically defined situations to expand clinically relevant hPSCs at low cost.
Environmental cues
To date, environmental cues also serve an essential role in the hPSC culture, comprising cues from physical and physiological environments which promote hPSC growth, for example, humidity, acidity, temperature, osmosity, cell density, gas diffusion exchange, and modes of multicellular associations. Among these factors, consumption of oxygen and the modes of multicellular associations are the two most important.
We offer standard and customized iPSC culture services including mycoplasma testing and FBS lot evaluation. We are experienced with almost all types of culture formats such as traditional plates, flasks, roller bottles, and spinner vessels. Following are some leading services available in Creative Biolabs. We offer customized services to meet every customer's need.
With professional scientists devoted themselves in iPSC culture, Creative Biolabs is dedicated to providing the first class iPSC culture services for our customers. Our services will contribute greatly to the success of your iPSC programs. Please contact us for more information and a detailed quote.
Features of Our Services
Our company's iPSC culture service offer various high-quality features that are bespoke to cater to different research needs.
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Detailed protocol - We provide complete and unambiguous protocols for initiating, maintaining and expanding iPSCs which helps in clear instructions and optimum output in research.
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Custom culturing – Depending on the unique needs of each researcher or organization, we offer custom iPSC culture services that conform to specific requirements, such as those dictated by a certain research project.
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High-quality culture media - To ensure the viability and maintain the pluripotency of iPSCs, we use high-quality, specialized culture mediums. This also includes feeder-free and xeno-free culture conditions to meet varying research demands.
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Reproducibility and automation - We assure reliable and reproducible culture conditions to promote consistent research results. To enhance scalability and reproducibility, we employ automated platforms in the iPSC culture process.
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High standard quality control - Every batch of iPSCs goes through stringent quality control tests to ensure the highest standards of pluripotency, sterility and mycoplasma. We even analyze the genomic stability of each line using karyotype testing.
By utilizing our iPSC reprogramming services, researchers and companies can accelerate research and development work and contribute to breakthroughs in regenerative medicine, disease modeling, and drug discovery. Our team remains at your disposal for the duration of the project, providing timely updates and addressing any issues or clarifications you may need.
FAQs
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Q: Can you customize the growth conditions according to my experimental requirements?
A: Yes, we can certainly cater to your specific needs. You can provide your desired growth conditions and our experienced technicians will adjust the stem cell culture conditions accordingly to meet the requirements of your experiment.
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Q: How quickly can you prepare the culture for my experiment?
A: The preparation time for iPSC cultures can vary depending on the specifics of your experiment. However, we typically require a minimum of two weeks for establishing and expanding a healthy, high-quality iPSC culture.
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Q: Can I track the progress of my iPSC culture preparation?
A: Certainly. We provide regular updates on the status of your iPSC cultures and you will be kept informed about the cell growth, differentiation, and quality checks.
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Q: How are the iPSC cultures packaged for shipment?
A: We ensure that the cultures are safely packaged for shipment while preserving the viability of iPSC cells. The cells are typically shipped in a sealed, insulated package with temperature control to ensure the cells remain viable during transit.
Scientific Resources
References
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Chen, K. G., (2014). “Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics.” Cell stem cell, 14(1), 13-26.
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Li, Y., (2017). “Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications.” Biofabrication, 9(3), 032001.
For Research Use Only. Not For Clinical Use.
