Induced pluripotent stem cells (iPSC) are cells derived from somatic cells with a number of defined factors. They served an important role in cell and tissue modeling, drug screening, and may also in future regenerative therapies. The characteristics of iPSC are similar to embryonic stem cells, thus similar methods are developed to characterize and differentiate them. With years of exploration in iPSC development, Creative Biolabs is dedicated to providing several characterization services of iPSC pluripotency for customers all over the world.
To characterize the pluripotency of iPSC, a class of defined tests can be used, and usually iPSC are characterized with the criteria applied to ES cells. Due to the differences between iPSC and ES cell expression patterns, their characteristics also not exactly the same. There are a variety of features assessed by characterization, such as cell and colony morphology, the expression of surface markers and other antigens, growth rate, the expression of pluripotency marker genes, enzyme levels, methylation statuses, as well as differentiation into somatic cell types in vitro and in vivo. Besides, among numerous pluripotency markers, OCT3/4 and Nanog are considered to be archetypal pluripotent stem cell markers. Moreover, it has demonstrated that OCT3/4 and SOX2 work together via feedback loops regulating both their own transcription and other pluripotency-associated genes.
Fig 1. Immunofluorescence staining for pluripotency markers. (Lampuoti, J. 2013)
Creative Biolabs is dedicated to providing several viable and cost-effective characterization methods for iPSC pluripotency. Usually, these approaches are based on RNA extraction, cDNA transcription, polymerase chain reaction, and immunofluorescence staining. Based on our well established iPSC platform, we are confident in offer the first class characterization services to contribute greatly to your project's success. Our services include but are not limited to items.
Markers to distinguish pluripotent and non-pluripotent cells now available for pluripotency marker assays in Creative Biolabs are showing in table 1.
Table 1. Gene and antigen markers analyzed for iPSC pluripotency. (Lampuoti, J. 2013)
Marker | Analyzed expression | Role/type |
c-Myc | Gene | Expression amplifier, reinforcing the pluripotent state |
Nanog | Gene, antigen | Establishment of induced pluripotency, resisting differentiation |
OCT3/4 | Gene, antigen | Pluripotency maintentance and induction |
Rex1 | Gene | Transcription factor (zinc finger protein), regulated by OCT3/4, SOX2, & Nanog |
SOX2 | Gene, antigen | Pluripotency maintentance and induction |
SSEA4 | Antigen | Stage-specific embryonic antigen, cell surface glycolipid |
TRA 1-60 | Antigen | Stem cell surface keratan sulfate antigen epitope |
TRA 1-81 | Antigen | Stem cell surface keratan sulfate antigen epitope |
With professional scientists devoted themselves in iPSC Pluripotency Characterization, Creative Biolabs is dedicated to providing the first class characterization services of iPSC pluripotency for our customers. Please contact us for more information and a detailed quote.
Our company's iPSC pluripotency characterization services offer a comprehensive repertoire of features to ensure your iPSCs are properly characterized, ensuring their pluripotency and usability in different research contexts.
Reference
For Research Use Only. Not For Clinical Use.