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Mesenchymal Stem Cells (MSCs) Differentiation Services

Overview Service Features FAQs Scientific Resources Related Services

Mesenchymal stem cells (MSCs) are undifferentiated multipotent cells that possess great potential in tissue regeneration and cell-based therapy due to their multipotency. Application of MSCs requires their isolation and direction of their differentiation into the appropriate lineage. Creative Biolabs offers customized services for reliable in vitro induction of MSCs into osteogenic, adipogenic, muscular, and chondrogenic lineages.

Differentiation Potential of MSCs

MSCs have the ability to differentiate into a large number of specialized cell types following specific signals (e.g., Wnt and redox signals, microRNAs, reactive oxygen species). These include mesodermal (osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes), ectodermal (neuronal cells) and endodermal (hepatic, pancreatic, respiratory epithelium) lineages. Moreover, a fully differentiated cell from one lineage can switch into another mature cell type of MSCs, which is a phenomenon called genetic reprogramming or transdifferentiation. As a result, MSCs derived from different tissues can be readily isolated and expanded for both basic and biomedical research. It has to be noted that MSCs from different tissues have exhibited differences in their differentiation capacity and regeneration potential. Besides, increased age of donor and passaging of cultured MSCs also decrease differentiation potential.

Differential potential of MSCs. Fig.1 Differential potential of MSCs.

Services at Creative Biolabs

Our services include the differentiation of MSCs into various cell types for studying their therapeutic potential in different diseases and conditions.

  • Our MSCs are sourced from reliable and ethical sources and have been extensively characterized for their quality and potency. We offer a variety of differentiation protocols tailored to the specific needs of our clients, including osteogenic, chondrogenic, adipogenic, and neurogenic differentiation.
  • Our team of experts has years of experience in MSC differentiation and will work closely with clients to develop a customized differentiation strategy that meets their research goals. We provide detailed protocols, guidance, and support throughout the differentiation process to ensure successful outcomes.
  • Our services can be used for a wide range of research applications, including drug discovery, regenerative medicine, and disease modeling. By utilizing our services, researchers can accelerate their research and gain valuable insights into the therapeutic potential of MSCs in various clinical settings.

Differentiation into Adipogenic Lineages

The processes by which MSCs commit to the adipose lineage and differentiate into adipocytes is termed adipogenesis. This process is triggered by signaling factors that induce conversion of MSCs to preadipocytes that differentiate into adipocytes. Thereafter, exposure of preadipocytes to differentiation inducers, such as insulin-like growth factor1, glucocorticoid, and cyclic AMP, triggers the expression of adipocyte genes and the production of adipocyte phenotype. With the use of appropriate cultures, Creative Biolabs can help induce the differentiation of your MSCs into both white and brown adipocytes.

Differentiation into Osteogenic Lineages

Manipulations of MSCs toward osteogenic lineage differentiation to form bone is a focused area in recent years. To help facilitate these research areas, Creative Biolabs focuses on providing MSCs osteogenesis differentiation system for in vitro studies. Optimal culture conditions, including many selective, temporally specific growth factors (BMP-2 and VEGF), are allowed in our differentiation protocols to enhance osteogenesis differentiation efficiently.

Differentiation into Chondrogenic Lineages

The capabilities of MSCs to differentiate into chondrogenic lineages make it a promising approach for cartilage repair and regeneration. The chondrogenic differentiation of MSCs is regulated by many factors and thus it relies on exposure to specific reagents (mainly inductive morphogens) to push MSCs to develop into specific phenotypes. Creative Biolabs has rich experience in promoting the chondrogenic differentiation of MSCs and enhancing the chondrogenic potential of dedifferentiated chondrocytes.

Overall, our services offer a convenient and reliable solution for researchers looking to explore the differentiation potential of MSCs in their research projects. Contact us today to learn more about how we can support your research endeavors.

Features of Our Services

  • Amenable to the research of different cellular applications and assay endpoints (e.g., bioengineering, tissue repair, etc)
  • Sufficient cell populations with the desired functionality and phenotype
  • Ongoing technical support and a range of tailored media to ensure the optimal culture
  • Customized services and individualized support to suit specific research requirements

Creative Biolabs conducts custom-specific contract research based on years of experience gained in a number of successful development projects. We can provide both individual service modules and full packages of services. Our state-of-the-art technologies and highly-qualified personnel guarantee that we can deliver maximum quality and flexibility in all phases of the product lifecycle. Please feel free to contact us for more information.

FAQs

  • Q: What differentiation lineages do you offer for MSCs?
    A: We offer differentiation services for several lineages, including osteogenic, adipogenic, and chondrogenic lineages. Additionally, we can differentiate MSCs into myogenic, neurogenic, and hepatogenic lineages based on your research requirements. Our protocols are optimized to provide reliable and reproducible results, ensuring that your MSCs achieve the desired phenotype with high efficiency and purity.
  • Q: How long does the differentiation process typically take?
    A: The duration of the differentiation process depends on the specific lineage and MSC source. Generally, osteogenic and adipogenic differentiation can take 2-3 weeks, while chondrogenic differentiation may require up to 4 weeks. More specialized lineages, such as neurogenic or hepatogenic, might take longer. We provide an estimated timeline during the initial consultation and keep you updated throughout the process.
  • Q: Can you provide differentiation services using MSCs from different species?
    A: Yes, we can differentiate MSCs from various species, including human, mouse, rat, and other animal models. Our expertise extends across different species to support a wide range of preclinical and translational research studies. If you have specific requirements for a particular species, we can customize our protocols to meet your research needs.
  • Q: What support do you offer after the delivery of differentiated MSCs?
    A: We offer extensive post-delivery support to ensure the successful integration of our differentiated MSCs into your research. This includes technical support for thawing and culturing the cells, troubleshooting any issues that may arise, and providing additional documentation or data as needed. Our team is available to answer any questions and assist you throughout your research project to ensure the best possible outcomes.
  • Q: Can you customize differentiation protocols based on specific research needs?
    A: Absolutely! We understand that different research projects may require unique differentiation conditions and protocols. Our team of experts can work closely with you to customize differentiation protocols tailored to your specific needs. Whether it's adjusting the culture medium, growth factors, or differentiation time points, we strive to provide a service that aligns perfectly with your research objectives.

Scientific Resources

For Research Use Only. Not For Clinical Use.