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Osteogenesis Differentiation of MSC Service

Overview Service Features Published Data FAQs Scientific Resources Related Services

The stem cell team at Creative Biolabs offers world-class expertise in the generation and differentiation of mesenchymal stem cells (MSCs) and uses this to provide solutions for your MSC needs. Our full-service packages are designed to meet the clients' project and specific requirements for osteogenesis differentiation of MSC.

MSC Osteogenic Differentiation in Health

MSCs are a population of stromal cells present in the bone marrow and most connective tissues, capable of differentiating into mesenchymal tissues such as bone and cartilage. MSCs from different sources are not necessarily equivalent. Indeed, they have shown significant functional differences and most certainly require specific optimal culture conditions and differentiation protocols for osteogenesis differentiation. MSCs can be used for differentiation into osteogenic lineages to form bone. The process involves three distinct stages from the commitment of osteoprogenitor cells, pre-osteoblasts differentiation, and mature osteoblasts formation.

Osteogenic differentiation of MSCs. Fig.1 Osteogenic differentiation of MSCs.1

  • Regulation of Osteogenic Differentiation
    • Runt-related transcription factor 2 (Runx2) and the transcription factor osterix (Osx) can initiate and continue osteogenesis.
    • Bone morphogenetic protein 2 (BMP2) and FBJ murine osteosarcoma viral oncogene homolog B (FosB) increase osteogenesis and inhibit adipogenesis.
    • Glycerophosphate and dexamethasone are used for the achievement of osteogenic differentiation in vitro.
    • Increased expression of alkaline phosphatase (ALP) and osteocalcin (OC) serve as markers of osteogenesis.
    • Some transcription factors, including CCAAT enhancer-binding protein beta (CEBPβ), gamma (CEBPγ), alfa (CEBPα), peroxisome proliferator-activated receptor-gamma (PPARγ), and Sp7 transcription factor (SP7) used for the control of the commitment of MSC differentiation to osteogenesis.

Service at Creative Biolabs

Creative Biolabs focuses on providing MSCs osteogenesis differentiation system for in vitro studies. Seasoned scientists at Creative Biolabs have set up a series of one-stop procedures to assist your cell model development with the highest efficiency and the best quality. We have established a cell culture system of MSCs that allows for osteogenic and adipogenic differentiation and trans-differentiation between both cell lineages. Also, selective, temporally specific addition of growth factors, such as BMP-2 and VEGF, appears to be an important strategy to enhance osteogenesis. We look forward to working with you in the future.

With our service, we provide biotechnology companies with a comprehensive platform to study the osteogenesis differentiation of MSCs. Our service includes MSCs isolation and expansion from various sources, such as bone marrow or adipose tissue. We then induce the differentiation of these MSCs into osteoblasts using specialized growth factors and culture conditions.

  • Throughout the differentiation process, we perform thorough characterization of the MSCs and osteoblasts using techniques such as flow cytometry, immunofluorescence, and gene expression analysis.
  • We also offer functional assays to assess the osteogenic potential of the differentiated cells, including Alizarin Red staining to visualize mineralization and osteogenic gene expression analysis.
  • We provide biotechnology companies with detailed reports summarizing the differentiation process and results, as well as recommendations for further research or applications.

Our service is intended for research use only and is conducted by our team of experienced scientists who have expertise in stem cell biology and osteogenesis.

Features of Our Service

  • A dedicated team of technicians and scientists
  • An individually tailored service
  • Access to reports and procedures
  • Fast turnaround time
  • Competitive pricing

Aided by our well-established platforms and experienced scientists, Creative Biolabs can provide osteogenesis differentiation service of MSC for our global customers. We prefer to selecting the best strategies according to your specific situation. Please feel free to contact us to learn more information or a detailed quote.

Published Data

Below are the findings presented in the article related to the osteogenesis differentiation of MSCs.

Crocin has rich pharmacological effects, but its role in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is not clear. Borui Li et al. explored the effect of crocin on osteogenic differentiation to provide a basis for its clinical application. They used cellular and animal experiments to analyze the osteogenic differentiation ability of hBMSCs.

When detecting the cell viability, the results showed that 100 and 1000 μmol/L crocin effectively inhibited hBMSCs’ viability. In animal experiments, the effects of crocin on the osteogenic differentiation of hBMSCs were investigated using 10, 25, and 50 μmol/L crocin as the therapeutic concentrations. ALP staining experiments showed that the ALP activity of crocin-treated cells was significantly higher than that of the control group. Alizarin red S staining also showed that more calcium nodules were formed in crocin-treated cells. Overall, the promotional effect of crocin on ALP activity and calcium nodule formation was enhanced with increasing concentration.

FAQs

  • Q: What types of MSCs can be used in your osteogenesis differentiation service?
    A: Our service can utilize MSCs derived from various sources, including bone marrow, adipose tissue, umbilical cord, and dental pulp. Each type of MSC has unique characteristics, and our team is experienced in handling and differentiating MSCs from these diverse sources. We can also accommodate specific requests if you have a particular MSC source in mind for your experiments.
  • Q: How long does the osteogenesis differentiation process take?
    A: The duration of the osteogenesis differentiation process typically ranges from 14 to 21 days, depending on the specific requirements and desired endpoints. This timeline includes initial MSC culture, induction, and differentiation stages, followed by thorough assessment and validation of osteogenic markers. We strive to optimize the timeline while ensuring high-quality results for your experiments.
  • Q: Can you customize the differentiation protocol based on my specific requirements?
    A: Absolutely! We offer customization of our differentiation protocols to meet the specific needs of your research. Whether you require adjustments in the induction medium composition, differentiation period, or specific assays for validation, our team is ready to tailor the service to align with your experimental goals. Please provide your requirements, and we will design a customized protocol accordingly.
  • Q: Can you work with my existing MSC cultures, or do you provide the cells as part of the service?
    A: MSC-derived osteoblasts have a wide range of applications, including bone regeneration studies, drug screening, disease modeling, and tissue engineering research. They are invaluable in studying osteogenesis, bone repair mechanisms, and the effects of various compounds on bone formation. Our service provides you with high-quality osteoblasts suitable for these advanced applications, supporting your research goals.
  • Q: What are the potential applications of MSC-derived osteoblasts generated through your service?
    A: We are flexible and can work with your existing MSC cultures or provide MSCs as part of the service. If you have well-established MSC cultures, we can use them for the differentiation process. Alternatively, if you prefer, we can source high-quality MSCs from our trusted suppliers to ensure consistency and reliability in the differentiation process.

Scientific Resources

Reference

  1. Infante, Arantza, and Clara I. Rodríguez. "Osteogenesis and aging: lessons from mesenchymal stem cells." Stem cell research & therapy 9.1 (2018): 1-7. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.