Magic™ Cell-Free Membrane Protein Expression Kits

E. coli Lysate Mammalian Cell Lysate Wheat Germ Lysate Insect Cell Lysate Rabbit Reticulocyte Lysate Plant Cell Lysate

Magic™ Cell-free Membrane Protein Expression Kit Cell-free Membrane Protein expression is the production of recombinant proteins in solution using biomolecular translation machinery extracted from cells of different species, such as E. coli lysate, wheat germ lysate, plant cell lysate, mammalian cell lysate, rabbit reticulocyte lysate, insect cells lysate. Creative Biolabs has developed a series of cell-free expression systems for high-quality, large/medium-scale membrane protein expression. We provide the most advanced Cell-free Membrane Protein expression kits for our global clients.

E. coli Lysate

• Facilitate the production of target proteins in high levels
• Simple protocol and easy setup with all necessary components
• The target proteins can be used in various downstream applications
• Protein of interest can be synthesized and visualized in a few hours
• Low cost

Cat	Product Name	Expected Yield of Positive Control
MPCFK-1	Magic™ Cell-free Membrane Protein Expression in E. coli Lysate	0.75 mg/ml
MPCFK-1-1	Magic™ Simplified Cell-free Membrane Protein Expression in E. coli Lysate	0.45 mg/ml
MPCFK-1-2	Magic™ Cell-free Membrane Protein Expression in E. coli Lysate - Dialysis Mode	5-10 mg/ml

Mammalian Cell Lysate

• Synthesized in as little as 1 hour and easy setup with all necessary components in a single-tube reaction
• Provides high yield and functional protein
• Enables the synthesis of large proteins (such as over 150 kDa)
• Modifications such as glycosylation and phosphorylation could be easily obtained
• Translation enhancement factor included in the Accessory Proteins ensures the high translation level
• Various applications: high throughput screening, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity, and protein-protein interactions

Cat	Product Name	Expected Yield of Positive Control
MPCFK-2	Magic™ Cell-free Membrane Protein Expression in Mammalian Cell Lysate	0.75 mg/ml
MPCFK-2-2	Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate	0.1 mg/ml

Wheat Germ Lysate

• Membrane protein has been synthesized using proteoliposome
• No need to prepare reagents
• Reagents necessary for each reaction are already pre-packaged in tubes
• Synthesize proteins with just a few pipetting steps
• Target proteins can be confirmed through SDS-PAGE/CBB staining

Cat	Product Name	Expected Yield of Positive Control
MPCFK-3	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate with liposome	40 µg/ml
MPCFK-3-1	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate	0.1-0.2 mg/ml
MPCFK-3-2	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate for membrane protein expression screening	20 µg/ml
MPCFK-3-3	Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate - Bilayer Format	20-40 µg/ml
MPCFK-3-4	Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for Protein Production	20-40 µg/ml
MPCFK-3-5	Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for GST-tagged Protein Production	20-40 µg/ml
MPCFK-3-6	Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Production	20-40 µg/ml
Search for More Cell-free Membrane Protein Expression Kits in Wheat Germ Lysate

Insect Cell Lysate

• Convenient, quick, single-tube, coupled transcription/translation system
• Allows both transcription and translation to occur in a single reaction
• Can be scaled by changing the component volumes proportionally
• T7 RNA polymerase promoter
• Addition of labels to the reaction

Cat	Product Name	Expected Yield of Positive Control
MPCFK-5	Magic™ Cell-free Membrane Protein Expression in Insect Cell Lysate	0.1 mg/ml

Rabbit Reticulocyte Lysate

• Compared with conventional cell-based/Cell-free Membrane Protein synthesis platforms, RRL system exerts multiple superior features:
• Easily prepared
• Cap-independent translation
• Compatible with mammalian post-translational protein modification system
• Large proteins greater than 30 kDa can be expressed efficiently
• Compatible with high-throughput format for protein microarrays and other screening procedures

Cat	Product Name	Expected Yield of Positive Control
MPCFK-6	Magic™ Cell-free Membrane Protein Expression in Rabbit Reticulocyte Lysate	Based on RNA purity

Plant Cell Lysate

• High-yield: Up to 3 mg/mL expressed protein
• Easy to use: One-step reaction protocol
• Fast: High yield in just 48 hours
• Flexible: Express "difficult to produce" proteins, cytotoxic proteins, etc.
• Convenience: suitable for various membrane protein expressions

Cat	Product Name	Expected Yield of Positive Control
MPCFK-7	Magic™ Cell-free Membrane Protein Expression in Plant Cell Lysate	3 mg/ml

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

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