Bone-Targeted Exosome Modification Service

Overview Services Features FAQs

Overview

The skeletal system consists of bones, joints, and ligaments, including bone, cartilage, fibrous connective tissue, blood vessels, blood, and nerve tissue, and is essential for the structure and support of the body. If there is a problem with the skeletal system of the human body, it will have a great impact on many aspects such as movement, activity, and hematopoiesis. Common bone diseases include osteoporosis (OP), osteoarthritis (OA), and malignant tumors. Precision therapy of bone diseases generally requires invasive surgical procedures to implant drugs to maintain effective drug concentrations in bone. However, this invasive implant still lacks specific targeting. Therefore, specific drug delivery systems that actively target bone are very important. Exosomes are natural novel nano-delivery vehicles. The surface modification of exosomes can be artificially endowed with cell or tissue specificity. Creative Biolabs can construct engineered exosomes that can target bone, providing a platform and research ideas for this problem.

Services

Engineered Exosomes Modified with Bone-Targeting Peptides

Due to its high affinity to low-crystalline hydroxyapatite and calcium phosphate on the bone-forming surface, the validated bone-targeting peptide, a polypeptide consisting of 6 repeating aspartate-serine-serine sequences (DSS6), can be modified on exosomes for bone-targeted drug delivery. Phospholipid molecule-DSS6 can be inserted into the exosomal phospholipid bilayer through hydrophobicity, resulting in bone-targeted exosomes (DSS6-Exo) with DSS6 on the surface. Previous studies have shown that DSS6-Exo loaded with siRNA can promote the bone formation of osteoblasts and angiogenesis. This indicates that DSS6-Exo is expected to be used in the precise treatment of OP.

Concept diagram of human femoral osteoporosis. (Creative Biolabs AI)

Engineered Exosomes Modified with Cartilage -Targeting Peptides

The dense connective tissue formed by the extracellular matrix of chondrocytes has been an obstacle to drug delivery by exosomes. Excitingly, researchers have screened out a chondrocyte affinity peptide (CAP, sequence: DWRVIIPPRPSA) through phage display technology, which can transport drugs into chondrocytes. Furthermore, the researchers have achieved fusion expression of CAP and lysosome-associated membrane glycoprotein 2b (Lamp2b) to construct CAP-Exo targeting chondrocytes. CAP-Exo encapsulated with miR-140 can play the biological role of miR-140 in the deep chondrocyte region through joint injection, inhibit cartilage degradation proteases, and alleviate the progression of OA.

Concept diagram of inflammation of the cartilage between the femur and tibia in humans. (Creative Biolabs AI)

Features

High-efficiency bone-targeted delivery system
Advanced technology platform
Potential for clinical translation
Professional team support

Bone-targeted exosomes can specifically guide therapeutic drugs to target tissues, showing their great potential for clinical translation. Creative Biolabs is committed to the development and clinical transformation of exosome technology and can provide one-stop services for the construction, extraction, and efficacy verification of engineered exosomes. Please feel free to contact us with your needs. Our professionals will provide customers with personalized solutions and a full range of services.

FAQs

Q: What types of therapeutic agents can be delivered using these modified exosomes?

A: These modified exosomes can deliver a variety of therapeutic agents, including siRNA, miRNA, proteins, and other small molecules, specifically designed to promote bone formation, inhibit cartilage degradation, or support tissue regeneration.

Q: How are the exosomes modified with targeting peptides?

A: The exosomes are modified by inserting targeting peptides into their phospholipid bilayer through hydrophobic interactions or by engineering donor cells to fuse targeting peptides with exosome surface proteins. This process ensures that the targeting peptides are displayed on the exosome surface, enabling precise targeting to bone or cartilage tissues.

Q: What is the potential for clinical application of these bone-targeted exosomes?

A: These bone-targeted exosomes have great potential for clinical application because they can specifically guide therapeutic drugs to bone or cartilage tissues, enhancing treatment efficacy and reducing side effects. They represent a promising strategy for the precise treatment of bone and cartilage-related diseases.