Disease-Targeted Exosome Modification Services
Overview Services Features FAQs
As an expert team in the exosome research and development field, Creative Biolabs has accumulated extensive project experience to provide exosome targeting solutions, whether involving targeting tissues and organs, or targeting focal regions.
As natural vesicles that play a mediating role in cellular communication, exosomes are involved in a variety of biological functions under physiological and pathological conditions. In the development of targeted delivery strategies for therapeutic drugs, exosomes have been considered highly promising delivery tools due to their unique advantages such as reduced immunogenicity, enhanced tissue permeability, deformable cytoskeleton, and similarity to cell membranes. However, the weak targeting properties of natural exosomes and their susceptibility to rapid clearance in vivo have limited the effectiveness of their encapsulated drugs, prompting research to improve the targeting properties of exosomes.
Surface modification to fabricate engineered exosomes is a modality with practical applications, providing a solution for precise targeting of therapeutics to the lesion. Commonly used engineering modifications in recent years include chemical linkage of targeting ligands/homing peptides, genetic engineering to modify exosomal membranes, magnetic nanoparticle technology, electrostatic interactions and post-insertion, with no effect on the proteins that play a decisive role in the biological function of exosomes. Peptide modifications based on membrane proteins as anchoring scaffolds and modification strategies based on encapsulated magnetic particles and electrostatic ion pairs have been screened to obtain several engineering strategies capable of manipulating the molecular composition of exosomes, greatly improving the capture of exosomes in the target region and the targeted release of drug effects.
Fig. 1 Surface engineering of exosomes via genetic/biological manipulation or chemical modification.1
Of particular interest as novel methods capable of manipulating exosomal protein composition, exosome display technologies enable soluble, membrane-bound, transmembrane or multimeric ligands, or homing peptides to be efficiently expressed on the surface of exosomes in their natural conformation. Specifically, targeted exosomes with target ligands or homing peptides are isolated by constructing fusion proteins or plasmids in which ligands/homing peptides are inserted into transmembrane proteins and then modifying the fusion proteins directly on the surface of exosomes or from donor cells after transfection with plasmids. Exploring the potential use of exosomes in targeted gene therapy is made possible by the discovery of several peptides that may be directed to damaged tissues or organs by phage display and biopanning techniques.
Fig. 2 Approaches for engineering exosomes displaying targeting ligands.1
Exosome Targeting Services
It is particularly proposed that the selection of appropriate modifications based on a thorough understanding of molecular metastasis mechanisms and specificity in context has a critical impact on the establishment of optimal selective exosome uptake and intracellular distribution systems. Exploring the safe and effective engineering of modified exosomes is necessary due to potential risks such as immunosuppression and reversal of tumorigenesis. Creative Biolabs provides solutions for exosome targeting, including but not limited to:
Features
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Tailored Modification: Our team of experts will customize exosomes to target specific diseases or conditions for research, enhancing their potential.
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Precision Delivery: By modifying exosomes to target diseased cells or tissues, we ensure a more precise and effective delivery of therapeutic cargo.
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Enhanced Efficacy: When compared to typical drug delivery systems, disease-targeted exosome alterations can increase efficacy while decreasing potential negative effects.
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Expert Support: Our team of experienced scientists and researchers is dedicated to providing high-quality services and support throughout the modification process.
FAQs
Q: What types of diseases can be targeted with exosome modifications?
A: Exosome modifications can be tailored to target a wide range of diseases for research, including cancer, neurodegenerative disorders, inflammatory conditions, and more.
Q: What is the duration of the modification service?
A: Depending on the particular needs of the project, the timeframe for the exosome modification process may change. Our team will work closely with you to establish a timeline and keep you informed throughout the process.
Creative Biolabs has established a comprehensive platform for targeted exosome development including targeted peptide engineering technology, magnetic nanoparticle technology, and electrostatic interactions. By combining highly versatile delivery capabilities with sophisticated engineering modifiability, our team is able to deliver best-in-class exosome targeting solutions to advance exosome research and industrial translation. Please contact us to discuss your project.
Reference
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Liang, Yujie, et al. "Engineering exosomes for targeted drug delivery." Theranostics 11.7 (2021): 3183. Under Open Access license CC BY 4.0, without modification.
For Research Use Only. Cannot be used by patients.
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