Stem Cell-derived Exosome Advantage and Application
- Inducing Regeneration
Overview Services Features FAQs
Creative Biolabs reviews and summarizes the studies and corresponding mechanisms of action of SC-Exo applied to regenerate connective tissues such as bone, cartilage, skin, spinal cord, and tendons, and can provide related research services.
Overview
In a model of osteoarthritis, SC-Exo has the effect of stimulating matrix regeneration of chondrocytes and accelerating cartilage formation. Chondrocytes treated by SC-Exo showed enhanced migration, cartilage differentiation and mechanism synthesis. The bone regeneration-promoting effect of SC-Exo was achieved by transfection of overexpressed molecules of miR-375 or HIF-1α in donor stem cells, or by increasing donor cell culture stimulation conditions such as hypoxia and TNF-α. Regulation of bone-producing related molecules by SC-Exo has been screened by miRNA sequencing and differential analysis, including increased bone-producing miR-10b and miR-2861 and decreased anti-osteogenic miR-31 and miR-144.
During the proliferation phase of skin cells, SC-Exo from diverse sources has the ability to stimulate fibroblasts to migrate from nearby healthy tissues to the site of damage and create a variety of matrix proteins, including type I collagen and type III collagen, to aid in the development of scar tissue. For instance, by releasing the Jagged 1 protein and turning on the Notch pathway, human embryonic dermal MSC exosomes cause the production of type I/III collagen, elastin, and fibronectin mRNA. Exosomes regulate the ratio of type I to type III collagen synthesis by regulating the expression ratio of TGF-3 to TGF-1, and MMP3 to MMP1, which promotes scar-free skin wound recovery.
Effluents from stem cells promote spinal cord regeneration mainly related to inflammatory regulation. For example, exosomes interact with activated microglia to inhibit the formation of NLRP3 inflammasome and reduce the levels of pro-inflammatory TNF-α, IL-6, IFN-γ and granulocyte colony-stimulating factor, alleviating limb motor and sensory dysfunction in spinal cord injury.
Tendon injuries are common in physical activity and athletics, and SC-Exo is beneficial for tendon or ligament regeneration. In a regeneration study in a rat tendon injury model, treatment with bone marrow-derived stem cells promoted tendon healing and improved fibrous and tendon architecture in a dose-dependent manner in the rat achilles tendon. In a rabbit rotator cuff tear and repair model, local injection of adipose stem cells at the tendon-bone junction reduced fat infiltration, promoted tendon-bone healing, increased newly generated fibrocartilage, and improved the biomechanical properties of the tendon-bone junction.
Services We Can Offer
At the forefront of exosome research, we offer a comprehensive suite of services centered around stem cell-derived exosome technology. Our services include the isolation and purification of high-quality exosomes, tailored to meet the specific needs of your research applications.
We provide detailed characterization and analysis, utilizing cutting-edge techniques such as RNA sequencing, proteomics, and advanced imaging to ensure the highest standard of quality. Additionally, we offer customized exosome-based research solutions, designed to accelerate explore tissue repair and regeneration.
Our expert consultation services guide you through every step of your project, from experimental design to data interpretation, ensuring your success in harnessing the transformative potential of exosome technology. Whether for research or preclinical applications, our dedicated team is here to support and enhance your endeavors in regenerative medicine.
Fig. 1 A head-to-head comparison of cell therapy and cell-free therapy.1
Features
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Regenerative Properties: Stem cell-derived exosomes are rich in growth factors and cytokines that promote tissue repair and regeneration, accelerating healing processes in various tissues.
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Natural and Safe: Derived from natural stem cells, these exosomes are biocompatible and have a lower risk of immune rejection compared to synthetic alternatives.
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Anti-Inflammatory Effects: These exosomes possess anti-inflammatory properties, reducing inflammation and associated pain in damaged tissues.
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Enhanced Cell Communication: By enhancing cell-to-cell communication, stem cell-derived exosomes improve the coordination of regenerative processes, leading to more efficient tissue repair.
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Versatile Applications: Suitable for a wide range of medical conditions, including skin injuries, cardiac repair, neurodegenerative diseases, and musculoskeletal disorders.
Research on SC-Exo for regeneration is currently an active and hot area. Creative Biolabs is committed to ongoing development of research services related to the regeneration-promoting effects of SC-Exo. Please contact us for more information.
FAQs
Q: How do stem cell-derived exosomes induce regeneration?
A: These exosomes deliver regenerative molecules directly to damaged tissues, enhancing cellular repair, reducing inflammation, and stimulating new cell growth.
Q: How can research be conducted on stem cell-derived exosomes?
A: Research can be conducted by isolating and characterizing exosomes from stem cells, performing in vitro and in vivo studies to evaluate their regenerative effects, and using advanced techniques such as RNA sequencing, proteomics, and imaging to understand their mechanisms.
Q: Are stem cell-derived exosomes safe?
A: They are considered safe due to their natural origin and biocompatibility. They carry a lower risk of immune rejection compared to other treatments.
Q: How is stem cell exosome administered?
A: Exosomes can be administered through various methods, including topical application, depending on the target area.
Reference
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Jayaraman, Selvaraj, et al. "Stem cell-derived exosomes potential therapeutic roles in cardiovascular diseases." Frontiers in cardiovascular medicine 8 (2021): 723236. Under Open Access license CC BY 4.0, without modification.
For Research Use Only. Cannot be used by patients.
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