miRNA sequencing for EVs(extracellular vesicles) presents a rich source for the exploration of disease diagnostic and prognostic biomarkers and the identification of therapeutic targets for various diseases. Creative Biolabs is equipped with a cutting-edge and efficient molecular sequencing platform to provide miRNA sequencing services for EVs, supporting customers' miRNA research on EVs with high-throughput and sensitive sequencing features.

Enriched miRNAs in EVs

EVs miRNAs are involved in various biological processes based on vesicular transport, including intercellular information exchange, regulation of gene expression and modulation of immune responses. For example, MSCs-EVs carry a variety of miRNAs, which can play an influential role in regulating the biological activities of immune cells, such as proliferation, maturation, activation, differentiation, migration, and effects, by regulating the expression of important signaling pathway molecules in immune cells and activating or inhibiting related pathways. miRNA species that are linked to particular diseases or conditions can be found via EVs miRNA sequencing. To be specific,

• This method allows for the identification of miRNA species that are up- or down-regulated in a particular situation, which can shed light on the underlying biological processes behind the development of disease.
• The identification of miRNA species linked to drug resistance using this method can also aid in the creation of more powerful therapeutics.
• miRNA species that are related to particular cell types or tissues can be found via EVs miRNA sequencing, which can aid in the creation of more specialized treatments.

EV miRNA Sequencing Procedures at Creative Biolabs

1. Isolation of EVs: EVs can be isolated from cell culture supernatants or from biological samples using a variety of techniques such as ultracentrifugation, density gradient centrifugation, or affinity-based isolation. The suitable isolation protocol according to the nature of the sample can facilitate the subsequent enrichment of more EVs miRNAs.
2. Purification of miRNA: Once EVs have been isolated, the miRNA can be purified using techniques such as polyacrylamide gel electrophoresis, reverse transcription-polymerase chain reaction (RT-PCR), or immunoprecipitation.
3. Sequencing of miRNA: After the miRNA has been purified, it can be sequenced using high-throughput sequencing techniques such as next-generation sequencing (NGS) or microarray analysis.
4. Analysis of Sequencing Data: Once the sequencing data has been obtained, it can be analyzed using bioinformatics tools to identify miRNA expression patterns, identify potential biomarkers, and understand the functional roles of the miRNA in the cell.

Fig.1 Scheme of the miRNA NGS data analysis from data generation to the final result.¹

EV miRNA NGS Features

• Comprehensive miRNA Profiling: Our service provides a thorough analysis of microRNA (miRNA) content within extracellular vesicles, allowing for detailed profiling of these small RNA molecules.
• High Sensitivity and Accuracy: Utilizing advanced sequencing technologies, we ensure high sensitivity and accuracy in detecting even low-abundance miRNAs from extracellular vesicles.
• Customizable Analysis: Tailored bioinformatics analysis to suit specific research needs, including differential expression analysis, pathway analysis, and more.
• Expert Consultation: Access to expert consultation for experimental design, data interpretation, and recommendations for optimizing results.
• Rapid Turnaround Time: Efficient workflows ensure timely delivery of sequencing results, facilitating swift progression of your research.
• Quality Assurance: Strict quality control procedures are followed at every stage to guarantee accurate and repeatable data.

miRNAs are considered to be significant components in various molecules afflicting the EVs function. Creative Biolabs provides miRNA sequencing services for EVs, supporting clients in deciphering the key regulators involved in the pathogenesis of various diseases in EVs. Please contact us with your interest, and the response will be back within a short time.

FAQs

Reference

1. Tesovnik, T.; et al. Technological approaches in the analysis of extracellular vesicle nucleotide sequences. Front Bioeng Biotechnol. 2021, 9: 787551. Under Open Access license CC BY 4.0. The image was modified by revising the title.

• Whole Transcriptome Sequencing
• mRNA Sequencing
• lncRNA Sequencing
• circRNA Sequencing