Cell-Penetrating Intrabody Discovery Service
With our advanced cellular and molecular technologies, Creative Biolabs has developed a first-class platform for IntraMab Discovery.
Introduction of Direct Internalizing IntraMab Discovery
Direct internalizing intrabodies could target a variety of intracellular molecules to achieve therapeutic effects. They could be applied in the construction of immunotoxins, immunoliposomes, ADCs, and other targeted delivery architectures. As shown in Fig. 1, the scFv-based arginine fusion peptide and an immunoliposome molecule deliver the drug molecules into the cell to play a curative role.
Fig. 1 Drug delivery mechanisms of direct internalizing scFv-based strategies.1
Workflow of Direct Internalizing IntraMab Discovery Service
Creative Biolabs provides a high-throughput and top-level specific technology for the selection and characterization of cell-specific and directly internalizing intrabody for immunotherapies. The workflow is shown below, in Fig.2.
Features & Benefits
Creative Biolabs is proficient in the screening, identification, and characterization of cell-specific and directly internalizing antibodies.
- Proficient experimental design for high-throughput, cell line-specific, and directly internalizing antibody screens.
- Comprehensive validation and characterization of directly internalizing antibodies.
- Data analysis is based on online resources and software tools.
- Intuitively visual interpretation for experimental results.
- One-stop engineering and optimization service for the application of directly internalizing intrabody.
Frequently Asked Questions
Q1: What's your strategy to generate the internalizing intrabody?
A1: To generate directly internalizing antibodies, our phage display library will be prepared for the target cell line. There are three stages for the phage internalizing process, binding to the cell surface, endocytosis, and internalizing within the cells. After the phage binding screen phase, non-specifically or weakly bound phage will be washed out. After the endocytosis screen phase, the phages binding to the cell surface antigens and in the extracellular matrix will be removed. Only the internalized phages will be recovered from within the cell and used for the next round of selection. After a few rounds of selection and enrichment, internalized phage clones will be further analyzed by a series of identification and characterization assays.
Q2: How to ensure that the intrabody was not internalized because of the phage?
A2: According to the screen design, the phage binding phase of the screen will be controlled to allow phage binding without internalization. The endocytosis phase of the screen will be controlled to allow the ligand-induced internalization of receptors and avoid phage degradation within the cells. All these efforts will help guarantee the get the direct internalizing intrabody will not be affected by the display vector phages.
Creative Biolabs has established a professional, experienced, and dedicated team to help our clients get direct internalizing intrabody, which has a large potential in the research of targeted therapeutic areas.
Reference
- Safdari, Yaghoub, et al. "Use of single-chain antibody derivatives for targeted drug delivery." Molecular Medicine 22 (2016): 258-270. Use under Open Access license CC BY 4.0, without modification.
All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.
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