Cytoplasm Positioning Intrabody Development Service

At Creative Biolabs, Super™ Intrabody Development Platform is dedicated to providing the most professional intrabody screening services based on superior yeast two-hybrid or phage display techniques. Subsequently, we engineer the discovered intrabodies which are satisfying the research or therapeutic goal primarily. Meanwhile, our advance development platform is able to optimize customer’s product with strict validation tests and multiple parameters based on in silico analysis to achieve the best specificity, bioactivity and precise residence or anchoring. With the most superior intrabodies screened and validated at Creative Biolabs, we also offer versatile sophisticated ways to introduce the intrabodies’ sequences into a series of target cell lines or primary cells of a range of organisms, such as human, mouse, rat, etc.

Cytoplasm Positioning

Based on our superior eukaryotic expression systems or customized cells, the screened intrabody sequence is primarily transcripted into messenger RNA (mRNA) first. Then the mRNA is released from the transcriptional machine, recognized and decoded by ribosome located in the cytoplasm by eukaryotic protein synthesis machine. After the transfer RNA (tRNA), free amino acids, elongation factors and other components belonging to the protein synthesis machine are involved in, the primary intrabody polypeptide is produced and is ready for the subsequent structure maturation and post-translational modifications (PTM), such as glycosylation which is extremely important for intrabody, acylation, prenylation, lipoylation, phosphorylation, carbonylation, biotinylation, ubiquitination, etc. Finally, the product intrabody without any N-terminal or C-terminal signal peptide targeting for other structure or organelles is residing in the cytoplasm.

Since the physiological conditions of cytoplasm such as pH and oxidative value, are sophisticatedly different from the extracellular environment, the intrabody residing in the cytoplasm needs to endure the harmful changes with undisturbed bioactivity and productivity. To address the technical bottleneck, our gifted scientists have developed multiple creative strategies to directly overcome or elaborately bypass it. Thus, the Creative Biolabs proudly provides the first-rate intrabody services to produce the most superior intrabody with the highest bioactivity and specificity and outputs the highest yield with our multiple superior eukaryotic expression systems.

Fig.1 Cytoplasm localization of intrabodies.

Other Anchoring Modification Options provided by Creative Biolabs

Including the cytoplasm, Creative Biolabs can also develop specific positioning antibody for a variety of destination, which including but not limited to:

At Creative Biolabs, we are always eager to create evolutional strategy and develop more novel and advanced technology to add wings to our clients’ valuable projects. If you are interested in developing novel intrabodies, please do not hesitate to inquire us for detailed information.

All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.