Intracellular Antibody Development Platform

Creative Biolabs has accumulated extensive experience to offer intracellular antibody development service. Antibodies against intracellular targets have been very difficult to be developed, due to their large size, complex structure, and incompatibility with the intracellular environment. However, there are a lot of intracellular (e.g. nuclear, cytoplasmic, lysosomal, and mitochondrial) proteins that are key regulators of various biological and pathophysiological processes, and can serve as therapeutic targets. In the light that there is high demand but only limited capabilities in developing antibodies that target intracellular proteins, Creative Biolabs has been actively exploring novel technologies for intracellular antibody development.

Research professionals at Creative Biolabs have established two proprietary platforms: Super™ Intrabody Development and Super™ Transbody Development, to fill the gaps in the field. Transbodies (or called cell-permeable antibodies) are antibodies or antibody fragments that can penetrate the cell membrane and be internalized into the target cells, while intrabodies are a class of antibodies or antibody fragments that are expressed in the cells that bear the corresponding intracellular antigens. Both platforms can serve to develop antibodies with custom formats against intracellular targets of interest. We offer a versatile set of approaches to promote the generation of transbodies or intrabodies according to the clients’ specific requirements.

Various Technologies Facilitating the Development of Intracellular Antibody

The establishment of our intracellular antibody development platform replies on our broad range of available technologies, such as Hybridoma, Phage Display, Antibody Engineering, Antibody Modification, and Non-Aggregate scFv Screening.

Advanced Intracellular Antibody Characterization

Once the intracellular antibodies are produced, our scientists can offer a broad range of approaches to endow them with multiple particular or novel customized characteristics. The intracellular antibody characterization services including but not limited to:

• Molecular Weight Analysis
• Disulfide Analysis
• Affinity Measurement
• De novo Antibody Sequencing
• Endotoxin Analysis
• Antibody Purity Analysis
• Paratope Mapping
• Epitope Mapping

Other Features of the Intracellular Antibody Development Platform

• High-quality novel intracellular antibody discovery.
• Multiple host animal species available [e.g. mouse, rat, rabbit, human, non-human primate (NHP), sheep, llama, camel, dog, and chicken].
• Versatile strategies available for customers’ choices.
• State-of-the-art downstream engineering procedures (e.g. humanization, affinity maturation, Fc engineering, and custom conjugation).
• Fast turnaround time.
• Competitive pricing.
• Timely progress reports.

Please send us an inquiry for detailed information. Our company is proud to carry out the most relevant strategies and to propose contracts with guaranteed results for your projects. We would try our best to provide a customized proposal to meet your project requirements in the quality, timeline, and budget.

All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.