Intracellular Plasma Membrane-Anchored Intrabody Development Services

At Creative Biolabs, our most professional scientific teams create a novel bioinformatic strategy to automatically screen almost the whole SP libraries and modification database and references of Homo sapiens, Mus musculus, Saccharomyces cerevisiae, Arabidopsis thaliana, etc. Testing with our strict validations and optimizing with the most scientific parameters based on in silico analysis, our platform identifies the most advanced SP sequences for the anchor on the intracellular plasma membrane. Creative Biolabs provides the first-rate intracellular plasma membrane-anchored intrabodies with a sophisticated and balanced optimization strategy to ensure the high product quality and bioactivity.

Plasma Membrane Positioning

Since numerous critical proteins are able to anchor on the cytoplasmic side of the plasma membrane via specific modifications and shuttle back from the membrane to the cytoplasm or other organelles via cleavage of anchoring modification, our scientists have adopted the sophisticated natural modification and mechanism, and established multiple optimized modifications or other techniques to anchor customer’s protein or intrabody on the intracellular plasma membrane. Physiologically, the bioprocesses may facilitate or inhibit the interaction of proteins with the cell membrane and contribute to the normal functions of the proteins attached. Subsequently, these bioprocesses occurred in the intracellular membrane region widely play extremely important roles in cellular signal transduction, such as the normal or cancerous cell growth. With scientific accumulation and professional experience of Creative Biolabs, we are providing the following modifications which have been adopted widely.

• Fatty acylation: intrabody is covalently attached with fatty acids
• Prenylation: intrabody is covalently attached with hydrophobic isoprene polymers

Custom Intrabody Discovery and Development

At Creative Biolabs, starting with our optimized intrabody screening approaches (e.g. IAC technology, bacterial two-hybrid technology, and mammalian two-hybrid technology) to identify the suitable intrabody for the interest targets, our scientists are able to design the customized intrabody to locate specifically and attached covalently to the cytoplasmic side of plasma membrane via multiple modifications learned from nature. Meanwhile, our scientists can perform multiple strict validation tests on multiple parameters based on in silico analysis to achieve the best specificity and highest bioactivity.

At Creative Biolabs, we are keeping alert for any novel and advanced technology progress to assist our clients’ valuable projects. Our scientists are dedicated to overcoming almost every bottleneck in the discovery and development of specific intrabodies. If you are interested in developing novel intrabodies, please do not hesitate to inquire us for detailed information.

Other Anchoring Modification Options provided by Creative Biolabs

Including the intracellular plasma membrane, Creative Biolabs can also develop specific positioning antibody for a variety of destination, which including but not limited to:

All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.