Extracellular Plasma Membrane Positioning Antibody Development Service

At Creative Biolabs, Super™ Intrabody Production Platforms rely on a screen of the SP libraries and targeting-related modifications from Homo sapiens, Mus musculus, Saccharomyces cerevisiae, Arabidopsis thaliana, etc. Subsequently, we identify the most suitable SP sequence or modification and optimized with strict validation tests and multiple parameters based on in silico analysis to achieve the best specific SP or modification with proper location and robust expression. Eventually, Creative Biolabs provides versatile targeting services with a balanced optimization strategy to ensure accurate anchoring with high product quality and bioactivity.

Extracellular Plasma Membrane Positioning

The scientists of Creative Biolabs has optimized the extracellular membrane anchoring with glycosylphosphatidylinositol linkage (GPI), which is a complex molecular group attached to the protein’s C-terminal carboxyl group via an amide linkage. Basically, the GPI complex consists of several main components interconnected: a phosphoethanolamine, a linear tetrasaccharide (composed of three mannoses and a glucosaminyl), and a phosphatidylinositol, which is glycosidically linked to the non-N-acetylated glucosamine of the tetrasaccharide. The whole modification occurs through a GPI-transamidase complex. Eventually, the fatty acid chains of the phosphatidylinositol are only inserted into the membrane and on the exterior surface of the plasma membrane.

In the normal physiological bioprocess, GPI modification exhibits an important role in the embryogenesis, development, neurogenesis, immune system and fertilization. Meanwhile, it is also associated with the membrane microdomains, transient homodimerization or apical sorting in polarized cells. Since the sugar residues in the tetrasaccharide and the fatty acid residues in the phosphatidylinositol group largely vary according to the very specific protein, the great diversity endows the GPI proteins with a wide range of biological functions. For example, the IZUMO1R/JUN0 (named after the Roman goddess of fertility) on the egg plasma has an essential role in sperm-egg fusion. Thus, Creative Biolabs is able to optimize the GPI modification with our decades’ professional experiences and a large database of detailed information on hundreds of natural GPI modification. From here, our designing protocol could test customer’s product with multiple valid parameters and localize intrabody more precisely.

Other Anchoring Modification Options provided by Creative Biolabs

At Creative Biolabs, our scientists are dedicated to designing the most creative and effective GPI on customer’s intrabodies and resulting in anchoring on the extracellular plasma membrane. Despite the simple GPI modification, we also provide customized combination of the secretory signal and other membrane anchoring modification to achieve the extracellular plasma membrane anchoring.

Fig. 1 Extracellular plasma membrane-anchored intrabody via GPI modification.

Including the extracellular plasma membrane, Creative Biolabs can also develop specific positioning antibody for a variety of destination, which including but not limited to:

All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.