Intracellular Functional SdAb Discovery Service

Creative Biolabs offers a full line of the best intracellular functional sdAbs and endows our products with multiple optimizations and modifications for varied requirements, such as specific intracellular residences and extracellular space via fusion of versatile signal peptides. Since our Super™ Intrabody Development Platform has accumulated the most professional experience for decades, our scientists are dedicated to developing the first-rate functional sdAb for either research or pharmaceutical purposes in the world.

What is the single domain antibody?

An intracellular functional single domain antibody (sdAb) is actually an antibody fragment consisting of a single monomeric variable domain of a heavy-chain Ab or of a common IgG with an MW of only 12-15 kDa (about 110 amino acids). The original discovery of sdAb is immunoglobulin new antigen receptor (IgNAR) of cartilaginous fishes (e.g. sharks). With the accumulation of research, the scientists have found heavy-chain Abs only in camelids (dromedaries, camels, llamas, and alpacas) among mammals.

Characteristics of single domain antibody

• Specific as a regular antibody or even more robust.
• More heat-resistance and stability in detergents and high concentration of urea.
• More solubility in water originated from camelid and fish, owing to the CDR3, which forms an extended loop covering the lipophilic site that normally binds to a light chain.
• Bulk production in bacteria or other expression systems due to the small size and single chain.

At Creative Biolabs, our scientists are dedicated to optimizing almost every parameter to maximize the advantages of our intracellular functional sdAb. Currently, sdAbs are undergoing rapid development in multiple pharmaceutical areas (Alzheimer’s disease, cancer, acute coronary syndrome, etc.) owing to their small size, simple production, and high affinity.

Biotechnological and diagnostic application

• Chromobody (fusion of a fluorescent protein): recognize and trace targets in different compartments of living cells for novel functional studies.
• GFP-nanotrap (couple of an anti-GFP sdAb to a monovalent matrix).
• Crystallization chaperones: reduce the conformational heterogeneity by binding and stabilizing just a subset of conformational states as auxiliary protein, meanwhile, mask surfaces interfering.
• Diagnostic biosensor: outperformance owing to the more dense couple on the biosensor surface, such as hPSA (human prostate-specific antigen) sensor.

One-stop solution intrabody production at Creative Biolabs

Creative Biolabs provides versatile strategies to generate desired sdAbs. Due to the different properties and advantages, our scientists are pleased to tailor the most appropriate system and optimization to meet our customer’s demands. Our scientists are dedicated to constructing high-quality libraries, performing the best Y2H, and detecting every single potential sdAb. Since Creative Biolabs has years of experience, our labs have developed multiple Y2H based platforms and constructed hundreds of particular libraries, successfully achieved thousands of specific sdAbs to boost our global customers’ research. With the extensive experience and advanced platform of Creative Biolabs, researchers achieve the best service and the most optimized products to conquer more scientific puzzles.

Please do not hesitate to inquire us to learn more details of developing your specific intracellular sdAbs.

All of our antibody products and services can only be used for preclinical research studies. Do not use them on humans.