Biotin-Liposome (CAT#: CLBD026LY)

Description
Biotin-Liposome is Liposome with the biotin group. Biotinylated liposomes can be conjugated noncovalently with streptavidin through either direct interaction with the protein/antibody conjugated to streptavidin or by coupling with other biotinylated proteins using streptavidin as a bridging molecule. Both avidin and streptavidin form strong noncovalent bond with biotin.
Lipid Composition
L-alpha-Phosphatidylcholine, Cholesterol, 18:1 Biotinyl PE
Buffer
PBS
pH
7.4
Storage
Store in dark at +4°C and do not freeze
Size
100 nm
Quantity
5mL (available in lyophilized powder)
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DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. How can biotin liposomes be used in cancer therapy?

    Biotin liposomes can be used to target cancer cells that overexpress biotin receptors, delivering drugs directly to the tumor site. This reduces the side effects associated with systemic distribution of cytotoxic drugs and improves the efficacy of the treatment by concentrating the therapeutic agent in the tumor environment.

  2. What are the advantages of using biotinylated liposomes over conventional liposomes?

    Biotin liposomes are specialized liposomal formulations that include biotin on their surface. This biotinylation enables specific binding to avidin or streptavidin, enhancing targeted delivery to cells expressing biotin receptors. This specificity makes biotin liposomes highly effective for targeted therapeutic delivery, particularly in cancer treatment where precision is crucial.

  3. Can biotin liposomes be used for purposes other than drug delivery?

    Yes, biotin liposomes are versatile and can be employed in various biomedical applications including gene delivery, vaccine adjuvant systems, and diagnostic imaging. Their biotin modification allows for the conjugation of various biomolecules, which can be tailored for specific research or clinical needs.

  4. What are the considerations for preparing biotin liposomes in a laboratory setting?

    Preparing biotin liposomes involves careful consideration of lipid composition, biotin conjugation, and encapsulation efficiency. Techniques such as thin-film hydration followed by extrusion or sonication are commonly used. Ensuring the correct biotin to lipid ratio and optimizing the encapsulation process are crucial for maintaining functional integrity and achieving desired targeting properties.

  5. How do storage conditions affect the stability of biotin liposomes?

    Biotin liposomes should be stored at controlled temperatures, typically 4°C, and protected from light to maintain their stability and prolong shelf life. It is important to prevent freeze-thaw cycles, which can disrupt the liposomal structure and affect their functional properties​

Biotin-Liposome-fig1


Drawing depicting the ILPCR assay format

The study focuses on the development of an ultrasensitive quantitative antigen detection system called Immunoliposome-PCR (ILPCR). This innovative method employs biotin-labeled polyethylene glycol (PEG) phospholipid conjugates integrated into liposomes as detection reagents. The liposomes encapsulate reporter DNA and are coupled to a biotin-labeled secondary antibody via a NeutrAvidin bridge. This system enhances the sensitivity and specificity of antigen detection, enabling the accurate quantification of antigens at very low concentrations.
Experimental results demonstrate the effectiveness of the biotin liposome detection reagent. Liposomes, approximately 120 nm in diameter, each containing about 800 accessible biotin receptors and 220 encapsulated reporters, were utilized to quantify carcinoembryonic antigen (CEA) in human serum. The ILPCR assay exhibited a linear dose-response curve from 10^-10 M to 10^-16 M CEA, with a detection limit of 13 fg/mL. This sensitivity surpasses traditional clinical assays by 1,500 times. Additionally, the study highlights the assay's reproducibility and repeatability, with coefficient of variance values below 6% for repeatability and below 2% for reproducibility. The research underscores the potential of ILPCR as a highly sensitive and reliable method for antigen detection, providing significant advancements over conventional techniques.

He, J., Evers, D. L., et al. Immunoliposome-PCR: a generic ultrasensitive quantitative antigen detection system. Journal of nanobiotechnology. 2012, 10: 1-17. Under Open Access license CC BY 2.0, without modification.

  • Superior Performance in Targeted Drug Delivery
    Creative Biolabs' biotin liposomes offer unparalleled precision in drug targeting, significantly enhancing therapeutic outcomes in cancer research compared to competitors.
  • Creative Biolabs' Pioneering Technology
    I highly recommend Creative Biolabs for their cutting-edge biotin liposomes which have proven essential in my advanced drug delivery studies.
  • Enhanced Bioavailability
    The biotin liposomes significantly improve drug bioavailability, making them indispensable for my pharmacokinetic studies.
  • Revolutionary Impact on Molecular Delivery
    These liposomes have revolutionized the way we deliver molecules across biological membranes, offering both versatility and efficiency.
  • Creative Biolabs' Commitment to Innovation
    Creative Biolabs' biotin liposomes are at the forefront of research technology, providing tailored solutions that significantly boost research efficacy.

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