Cyanur-Doxorubicin Liposome (PEGylated) (CAT#: CLBD012LY)

Description
This formulation is Doxorubicin Liposome (PEGylated) with the cyanur group. Proteins can be covalently coupled to the liposome via amine-reactive cyanur-groups, either directly to the vesicle surface using cyanuric chloride-activated DSPE (cyanur-DSPE) or to the distal ends of PEG-spacers using activated cyanur-PEG-PE (ammonium salt). Cyanuric chloride at the PEG terminus functions to link peptides, antibodies and other amine-containing biomolecules or nanoparticles via a nucleophilic substitution reaction under basic conditions. Antibodies or other proteins can be conjugated without any previous derivatization.
Lipid Composition
HSPC, Cholesterol, DSPE-PEG(2000), DSPE-PEG(2000)-Cyanur
Encapsulated Drug
Doxorubicin
Buffer
Borate buffer, pH 8.8
Storage
Store in dark at +4°C and do not freeze
Size
100 nm
Quantity
5mL (available in lyophilized powder)
Molecular Structure
Download
DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. Can antibodies be conjugated directly to the liposome without prior derivatization?

    Yes, antibodies or other proteins can be directly conjugated to the PEG terminus of the liposome without prior derivatizations.

  2. What is the molar concentration of the Cyanur-Doxorubicin Liposome (PEGylated)?

    The molar concentration is 21.58 mM.

  3. Why is Tris buffer not recommended in the conjugation process?

    Tris buffer contains amines, which can interfere with the amine-reactive cyanur-groups, thereby hindering proper conjugation.

  4. What kind of proteins can be covalently coupled to these liposomes?

    Any amine-containing biomolecules, such as peptides, antibodies, and nanoparticles, can be coupled.

  5. How does this liposome formulation improve the antitumor activity of doxorubicin?

    The targeted delivery and passive accumulation of the liposome in tumors enhance the antitumor efficacy of doxorubicin, reducing off-target effects.

Cyanur-Doxorubicin Liposome (PEGylated)-fig1


Calcein uptake by HeLa cells incubated with control and Tf-PEG liposomes and exposed to low-frequency ultrasound (LFUS).

This project explores the targeted effect of immunoliposomes on cancer cells. The researchers used cyanuric chloride as a coupling agent to conjugate transferrin (Tf) with PEGylated liposomes, thereby producing immunoliposomes, and evaluated their uptake in HeLa cells. The results showed that the uptake of calcein after treatment with Tf-PEG liposomes was significantly higher than that of the control liposomes, with this effect further enhanced following ultrasound treatment. In this study, cyanuric chloride proved to be an effective coupling agent for the preparation of immunoliposomes, which holds significant implications for targeted drug delivery.

AlSawaftah, Nour M., et al. "Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells." Scientific reports 11.1 (2021): 11589.

Distributed under Open Access license CC BY 4.0, without modification.

  • Superior Targeting
    Cyanur-Doxorubicin Liposome (PEGylated) allowed us to target cancer cells with unprecedented specificity.
  • Stable Conjugation
    The stable protein attachment to the liposome surface provided consistent results in our drug delivery experiments.
  • Optimized Delivery
    Using Cyanur-Doxorubicin Liposome significantly optimized the delivery and therapeutic impact of doxorubicin.
  • Exceptional Quality
    The quality of the Cyanur-Doxorubicin Liposome (PEGylated) from Creative Biolabs is exceptional, ensuring reproducible results.
  • Easy Conjugation Process
    The easy conjugation process allowed us to quickly and reliably attach antibodies to the liposome.

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For Research Use Only. Not For Clinical Use

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