This formulation is Doxorubicin Liposome (PEGylated) with the cyanur group. Proteins can be covalently coupled to the liposome via amine-reactive cyanur-groups, either directly to the vesicle surface using cyanuric chloride-activated DSPE (cyanur-DSPE) or to the distal ends of PEG-spacers using activated cyanur-PEG-PE (ammonium salt). Cyanuric chloride at the PEG terminus functions to link peptides, antibodies and other amine-containing biomolecules or nanoparticles via a nucleophilic substitution reaction under basic conditions. Antibodies or other proteins can be conjugated without any previous derivatization.
FAQsPublished Data Customer ReviewsRelated Sections
Can antibodies be conjugated directly to the liposome without prior derivatization?
Yes, antibodies or other proteins can be directly conjugated to the PEG terminus of the liposome without prior derivatizations.
What is the molar concentration of the Cyanur-Doxorubicin Liposome (PEGylated)?
The molar concentration is 21.58 mM.
Why is Tris buffer not recommended in the conjugation process?
Tris buffer contains amines, which can interfere with the amine-reactive cyanur-groups, thereby hindering proper conjugation.
What kind of proteins can be covalently coupled to these liposomes?
Any amine-containing biomolecules, such as peptides, antibodies, and nanoparticles, can be coupled.
How does this liposome formulation improve the antitumor activity of doxorubicin?
The targeted delivery and passive accumulation of the liposome in tumors enhance the antitumor efficacy of doxorubicin, reducing off-target effects.
Calcein uptake by HeLa cells incubated with control and Tf-PEG liposomes and exposed to low-frequency ultrasound (LFUS).
This project explores the targeted effect of immunoliposomes on cancer cells. The researchers used cyanuric chloride as a coupling agent to conjugate transferrin (Tf) with PEGylated liposomes, thereby producing immunoliposomes, and evaluated their uptake in HeLa cells. The results showed that the uptake of calcein after treatment with Tf-PEG liposomes was significantly higher than that of the control liposomes, with this effect further enhanced following ultrasound treatment. In this study, cyanuric chloride proved to be an effective coupling agent for the preparation of immunoliposomes, which holds significant implications for targeted drug delivery.
AlSawaftah, Nour M., et al. "Transferrin-modified liposomes triggered with ultrasound to treat HeLa cells." Scientific reports 11.1 (2021): 11589.
Distributed under Open Access license CC BY 4.0, without modification.
Superior Targeting
Cyanur-Doxorubicin Liposome (PEGylated) allowed us to target cancer cells with unprecedented specificity.
Stable Conjugation
The stable protein attachment to the liposome surface provided consistent results in our drug delivery experiments.
Optimized Delivery
Using Cyanur-Doxorubicin Liposome significantly optimized the delivery and therapeutic impact of doxorubicin.
Exceptional Quality
The quality of the Cyanur-Doxorubicin Liposome (PEGylated) from Creative Biolabs is exceptional, ensuring reproducible results.
Easy Conjugation Process
The easy conjugation process allowed us to quickly and reliably attach antibodies to the liposome.
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