Histidine as a type of Electron-rich ligand shows a relatively high affinity to bond with electropositive transition metal, Ni2+. NTA(Ni)-Liposome is Liposome with the Ni-nitrilotriacetic acid (NTA), which can be conjuagted to His-tagged proteins or peptides. The liposomes with Ni-NTA headgroups are combined with the His residues, typically at the N- or C-terminus of proteins, the proteins reversibly anchor to the liposomes.
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What is the primary application of NTA(Ni) liposomes in research?
NTA(Ni) liposomes are particularly useful for binding His-tagged proteins or peptides due to the Ni-nitrilotriacetic acid (NTA) which has a high affinity for histidine residues. This makes them ideal for applications involving protein purification, detection, or cellular delivery systems.
How do NTA(Ni) liposomes enhance the stability of conjugated biomolecules?
The presence of Ni-NTA on the liposome surface stabilizes His-tagged biomolecules by providing a chelated and oriented attachment, which can improve the bioactivity and durability of the conjugates in various biological assays.
Can NTA(Ni) liposomes be used in in vivo studies?
Yes, NTA(Ni) liposomes are suitable for in vivo applications due to their biocompatibility and the ability to deliver His-tagged proteins or peptides efficiently to targeted cells or tissues, thereby enhancing the effectiveness of therapeutic and diagnostic agents.
What are the key considerations for using NTA(Ni) liposomes in cell culture?
When using NTA(Ni) liposomes in cell culture, it is crucial to maintain optimal conditions to prevent non-specific binding. This includes controlling the concentration of imidazole in the medium, which can compete with His-tags for binding to the NTA(Ni).
How does the particle size of NTA(Ni) liposomes affect their function?
The size of NTA(Ni) liposomes can influence their biodistribution, cellular uptake, and clearance rates in vivo. Smaller liposomes may be more efficient in penetrating tissues and being taken up by cells, while larger particles might have prolonged circulation times.
Production and characterization of second-generation liposomes
The research paper focuses on the design and functional characterization of T helper liposomes conjugated with the HIV-1 envelope protein. These liposomes aim to enhance the humoral immune response against HIV by utilizing intrastructural help (ISH). The study highlights the construction of liposomes displaying a dense array of HIV-1 Env trimers on their surface and encapsulating T helper cell epitopes. Immunogold staining confirmed that the Env trimers retained their prefusion conformation, essential for eliciting a proper immune response. The use of NTA(Ni) liposomes facilitated the stable coupling of His-tagged Env trimers via Ni-NTA interactions and EDC/Sulfo-NHS crosslinking, ensuring a robust and precise antigen presentation. The study demonstrated that these Env-coupled liposomes effectively activated Env-specific B cells and promoted CD4+ T cell proliferation, crucial for a strong adaptive immune response. Moreover, the encapsulated peptides were efficiently presented by dendritic cells in secondary lymphoid organs, further validating the potential of these liposomes in vaccine development. The results suggest that this liposomal platform could serve as a customizable and GMP-scalable alternative to traditional virus-like particle (VLP) vaccines, providing a promising approach for future HIV vaccine strategies.
Damm, D., Suleiman, E., et al. Design and Functional Characterization of HIV-1 Envelope Protein-Coupled T Helper Liposomes. Pharmaceutics. 2022, 14, 1385. 2022. Under Open Access license CC BY 4.0, without modification.
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