Propionylation Specific Antibody Discovery Service

It is understood that challenges are presently encountered in the identification and characterization of protein propionylation, a post-translational modification (PTM) of significant import. Creative Biolabs' Propionylation-Specific Antibody Discovery Services facilitate the acquisition of antibodies exhibiting elevated specificity and sensitivity, thereby enabling the advancement of research endeavors through sophisticated antibody development platforms. Bespoke solutions, meticulously tailored to the unique exigencies of individual projects, are provided, thus ensuring the attainment of results that are both reliable and reproducible.

Background

Propionylation, a post-translational modification (PTM), involves the addition of a propionyl group (C₃H₅O) to lysine residues in proteins. This modification is similar to acetylation but introduces a larger acyl group, potentially leading to distinct structural and functional consequences. Propionylation has emerged as an important regulatory mechanism in various biological processes, including chromatin dynamics, gene transcription, and metabolism.

Fig.1 Strategies of propionylation research.¹

The enzymes responsible for adding propionyl groups are acyltransferases, while deacetylases can remove them. The balance between these enzymatic activities regulates the propionylation status of proteins. Dysregulation of protein propionylation has been implicated in various diseases, highlighting the importance of studying this modification. The ability to specifically detect and quantify propionylation is crucial for understanding its biological roles and pathological implications. This is where our Propionylation-Specific Antibody Discovery Services become essential. We provide the tools necessary to investigate this important PTM.

Antibody Types

Through our service, you can obtain both monoclonal and polyclonal antibodies, with rabbit, mouse, and sheep as host animals. The antibody types include:

• Site-Specific Anti-Propionylation Antibodies: These antibodies recognize propionylation at a specific lysine residue, allowing researchers to pinpoint the modification's precise location and elucidate its influence on protein-protein interactions, enzymatic activity, and protein stability, thus aiding in understanding its contribution to cellular signaling and regulatory networks.
• Pan Anti-Propionylation Antibodies: These antibodies recognize propionylation regardless of the specific lysine residue. They offer a broader perspective, proving useful for global analysis of protein propionylation levels and distribution across the proteome. Such antibodies facilitate the identification of trends and patterns in propionylation, revealing its changes in response to various stimuli, cellular states, or disease conditions, and can be instrumental in biomarker discovery and assessing its overall impact on cellular physiology.

Discovery Strategy

Our antibody development services are primarily achieved through the following three strategies:

• Monospecific Anti-Propionylation Polyclonal Antibody Production: This strategy involves immunizing animals (e.g., rabbits) with a carefully designed propionylated peptide antigen. The resulting polyclonal antibodies are then purified and characterized to ensure high specificity for propionylation. This approach is well-suited for generating antibodies against specific propionylation sites.

• Phage Display Strategy for Anti-Propionylation Monoclonal Antibody Discovery: Phage display is a powerful technique for generating monoclonal antibodies. A library of antibody fragments is displayed on the surface of bacteriophages, and those that bind specifically to the propionylated antigen are selected. This method allows for the rapid identification of high-affinity antibodies.

• Hybridoma Strategy for Anti-Propionylation Monoclonal Antibody Discovery: The hybridoma technology involves fusing antibody-producing B cells from immunized animals (e.g., mice) with myeloma cells to create hybridomas. These hybridomas produce monoclonal antibodies that are specific to the propionylated antigen. This is a classical and robust method for generating monoclonal antibodies.

Types of PTM

We provide propionylation specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

Creative Biolabs stands as the perfect collaborator for discovering antibodies that target propionylation. We offer:

• High Specificity and Sensitivity: Our antibodies are rigorously validated to ensure minimal cross-reactivity with other modifications, such as acetylation, and high sensitivity for detecting low levels of propionylation.
• Customizable Solutions: We offer flexible service packages tailored to your specific research needs, including antigen design, antibody production, and characterization.
• Expert Team: Our team of experienced scientists has extensive expertise in antibody development and PTM analysis, ensuring the highest quality results.
• Proven Track Record: Published data support the reliability and effectiveness of our antibody development services.

FAQs

Q: Can your antibodies distinguish between propionylation and other similar modifications?

A: Yes, our antibodies are designed and rigorously tested for high propionylation specificity and minimal cross-reactivity with modifications like acetylation. Contact us for detailed specificity data.

Q: What sample types are compatible with your antibodies?

A: Our antibodies work with various sample types, including cell lysates, tissue extracts, and purified proteins, though specific preparation protocols may be needed. Contact our team for recommendations.

Q: What are the advantages of using your propionylation-specific antibodies in my research?

A: Our antibodies enable accurate and specific detection of protein propionylation, providing deeper insights into its role in biological processes and disease, thus accelerating your research. Inquire now about application-specific benefits.

Q: How do your antibody discovery strategies compare to other methods?

A: We use phage display and hybridoma technology to generate high-quality antibodies. These methods offer advantages in specificity, affinity, and scalability over traditional approaches. Let us help determine the best strategy for your project.

Creative Biolabs offers comprehensive Propionylation-Specific Antibody Discovery Services to empower your research. Our high-quality antibodies, combined with our expertise in antibody development, enable you to accurately and specifically detect protein propionylation. Please contact us for more details.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.